通过斑点印迹法检测M2抗线粒体抗体比酶联免疫吸附测定法更具特异性。

Detection of M2 antimitochondrial antibodies by dot blot assay is more specific than by enzyme linked immunosorbent assay.

作者信息

Bargou I, Mankaï A, Jamaa A, Ben Jazia I, Skandrani K, Sfar H, Baccouche A, Ajmi S, Letaief A, Fabien N, Jeddi M, Ghedira I

机构信息

Department of Immunology, Research Unit (03/UR/07-02), Faculty of Pharmacy, Monastir, Tunisia.

出版信息

Pathol Biol (Paris). 2008 Feb;56(1):10-4. doi: 10.1016/j.patbio.2007.05.001. Epub 2007 Jun 28.

DOI:10.1016/j.patbio.2007.05.001

PMID:17604571

Abstract

AIMS

The objective of our study was, in one hand, to determine the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of ELISA and dot blot assay to investigate IgG M2 antimitochondrial antibodies (M2 AMA) and, on the other hand, to compare these results with those of indirect immunofluorescence technique (IIF).

METHODS

Sera from patients suffering from primary biliary cirrhosis (PBC) (n=55), systemic lupus erythematosus (n=21), celiac disease (n=30) and blood donors (n=75) were analyzed. M2 AMA were detected by ELISA and dot blot using pyruvate dehydrogenase purified from porcine heart and by IIF on cryostat sections of rat liver-kidney-stomach.

RESULTS

IIF was more sensitive (98%) than ELISA (93%) and dot blot (91%). The specificity of AMA for PBC using IIF, ELISA and dot blot reached 100%, 92% and 100%, respectively. The PPV of IIF, ELISA and dot blot was 100%, 93% and 100%, respectively. The NPV was 98% for IIF, 92% for ELISA and 91% for dot blot.

CONCLUSION

Dot blot, using purified pyruvate dehydrogenase, had a higher specificity than ELISA and may be useful in confirming the specificity of AMA in cases of doubt with IIF.

摘要

目的

本研究的目的一方面是确定酶联免疫吸附测定（ELISA）和斑点印迹法检测IgG M2抗线粒体抗体（M2 AMA）的敏感性、特异性、阳性预测值（PPV）和阴性预测值（NPV），另一方面将这些结果与间接免疫荧光技术（IIF）的结果进行比较。

方法

分析了原发性胆汁性肝硬化（PBC）患者（n = 55）、系统性红斑狼疮患者（n = 21）、乳糜泻患者（n = 30）和献血者（n = 75）的血清。使用从猪心中纯化的丙酮酸脱氢酶通过ELISA和斑点印迹法检测M2 AMA，并在大鼠肝-肾-胃的低温切片上通过IIF检测。

结果

IIF比ELISA（93%）和斑点印迹法（91%）更敏感（98%）。使用IIF、ELISA和斑点印迹法检测AMA对PBC的特异性分别达到100%、92%和100%。IIF、ELISA和斑点印迹法的PPV分别为100%、93%和100%。IIF的NPV为98%，ELISA为92%，斑点印迹法为91%。