Department of Organic Chemistry A Mangini, University of Bologna, Bologna, Italy.
Skin Res Technol. 2009 Nov;15(4):503-10. doi: 10.1111/j.1600-0846.2009.00399.x.
BACKGROUND/PURPOSE: Foot malodor is mostly due to short-chain fatty acids produced by bacterial metabolism of eccrine sweating. We aimed to develop a protocol for an objective (instrumental) efficacy evaluation of foot deodorant formulations.
Head-space solid-phase microextractions of target fatty acids from the feet of six healthy volunteers were analyzed by GC-MS. A comparative analysis of the treated vs. the untreated foot was performed in each subject after washing the feet with a physiologic solution and incubating at 36 degrees C for 24-72 h in tryptic soy agar growth medium.
Acetic, butyric, isobutyric and isovaleric acids were identified as the main contributors to foot malodor in the majority of volunteers. Propionic, valeric and isocaproic acids were also detected in some subjects. Comparative analysis according to the protocol developed showed a statistically significant (P<0.01) reduction of target fatty acids ranging from -26.6% to -77.0%.
The protocol developed is a convenient, sensitive and non-invasive method to test the efficacy of foot deodorant formulations in human volunteers.
背景/目的：足部异味主要是由于细菌代谢的汗液中的短链脂肪酸产生的。我们旨在制定一个足部除臭剂配方客观（仪器）功效评估的方案。
用 GC-MS 对 6 名健康志愿者足部的目标脂肪酸进行顶空固相微萃取分析。在生理溶液中清洗双脚后，在 36 度下培养 24-72 小时，在胰蛋白酶大豆琼脂生长培养基中，对每个受试者的处理后的足部与未经处理的足部进行比较分析。
在大多数志愿者中，乙酸、丁酸、异丁酸和异戊酸被确定为足部异味的主要贡献者。在一些受试者中也检测到丙酸、戊酸和异己酸。根据制定的方案进行的比较分析显示，目标脂肪酸的减少具有统计学意义（P<0.01），范围从-26.6%到-77.0%。
所制定的方案是一种方便、敏感和非侵入性的方法，可用于测试人体志愿者中足部除臭剂配方的功效。
