Richards R Geoff, Simpson Angharad E, Jaehn Katharina, Furlong Pamela I, Stoddart Martin J
AO Research Institute, Davos Platz, Switzerland.
ALTEX. 2007;24 Spec No:56-9.
A 3D load-providing culture system is presented, which enables human cancellous bone explants to be studied ex vivo. Bone integrity and activity was demonstrated during 2 to 3 weeks of culture. Viable osteocytes and bone specific markers could be identified. Culture conditions were improved using a serum free medium containing TGF-beta3. Therefore this system gives the opportunity to study bone biology, hormonal effects as well as biomaterial interactions ex vivo prior to animal studies.
本文介绍了一种三维负载培养系统，该系统能够在体外研究人松质骨外植体。在2至3周的培养过程中，证明了骨的完整性和活性。可以识别存活的骨细胞和骨特异性标志物。使用含有转化生长因子β3的无血清培养基改善了培养条件。因此，该系统为在动物研究之前在体外研究骨生物学、激素作用以及生物材料相互作用提供了机会。
