通过光谱电化学法测定嗜热栖热放线菌光系统II中初级质体醌电子受体Q(A)的氧化还原电位。
Redox potential of the primary plastoquinone electron acceptor Q(A) in photosystem II from Thermosynechococcus elongatus determined by spectroelectrochemistry.
作者信息
Shibamoto Tadao, Kato Yuki, Sugiura Miwa, Watanabe Tadashi
机构信息
Institute of Industrial Science, The University of Tokyo, 4-6-1, Komaba, Meguro-ku, Tokyo 153-8505, Japan.
出版信息
Biochemistry. 2009 Nov 17;48(45):10682-4. doi: 10.1021/bi901691j.
The redox potential of the primary plastoquinone electron acceptor Q(A), E(m)(Q(A)/Q(A)(-)), in an oxygen-evolving photosystem (PS) II complex from a thermophilic cyanobacterium Thermosynechococcus elongatus was determined to be -140 +/- 2 mV vs. SHE by thin-layer cell spectroelectrochemistry for the first time. The E(m)(Q(A)/Q(A)(-)) value obtained here together with the recently determined redox potential of pheophytin (Phe) a [Kato et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106, 17365-17370] yields -330 to -370 mV for the free energy change by electron transfer from Phe a(-) to Q(A) and provides a renewed picture for the energetics on the electron acceptor side in PS II.
首次通过薄层电池光谱电化学法测定了嗜热蓝细菌嗜热栖热放线菌放氧型光系统（PS）II复合物中初级质体醌电子受体Q(A)的氧化还原电位E(m)(Q(A)/Q(A)(-))，相对于标准氢电极（SHE）为-140±2 mV。此处获得的E(m)(Q(A)/Q(A)(-))值与最近测定的脱镁叶绿素（Phe）a的氧化还原电位[加藤等人（2009年）《美国国家科学院院刊》106，17365 - 17370]相结合，得出从Phe a(-)到Q(A)的电子转移自由能变化为-330至-370 mV，并为PS II中电子受体侧的能量学提供了新的图景。
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