Department of Pathology and Pediatrics, Center for Complementary Medicine Research, Child and Family Research Institute, University of British Columbia, Vancouver, BC, Canada.
J Ethnopharmacol. 2010 Feb 3;127(2):478-85. doi: 10.1016/j.jep.2009.10.010. Epub 2009 Oct 14.
To search for more effective treatment of ovarian cancer, we investigated the in vitro anti-proliferation activities of Devil's club (OH) root bark extracts, an important medicinal plant of North America, on cisplatin sensitive and resistant human ovarian cancer cell lines.
High performance liquid chromatography was used to provide the chemical profiling of the OH extracts. The anti-proliferation activities of OH extracts alone or in combination with cisplatin or paclitaxel were determined on four human ovarian cell lines by crystal violet assay. Flow cytometry and light microscopy were employed for cell cycle analysis, and also to detect apoptosis.
Our data showed that water, 70% ethanol, 100% ethanol, and ethyl acetate extracts of OH inhibited the proliferation of human ovarian cancer cell lines A2780, A2780CP70, OVCAR3, and OVCAR10 in vitro. The respective 50% inhibition (IC(50)) was estimated at 1/256, 1/74, 1/69, 1/53; 1/4156, 1/1847, 1/1029, 1/4530; 1/25,753, 1/3310, 1/3462, 1/5049; and 1/29,916, 1/2912 1/3828, and 1/4232 dilutions. Some combinations of non-cytotoxic dilutions (<IC(50)) of 70% ethanol OH extract with cisplatin and paclitaxel enhanced its anti-proliferation IC(50) on A2780 and A2780CP70 cells. Cell cycle analysis demonstrated that the effect of OH extract on cell cycle was dependent on the concentration tested, blocking cells in the S and G2/M phases. At low concentrations it induced cell death by apoptosis, while at high concentrations, it kills cells by necrosis.
Our data showed that OH extracts exhibited significant anti-proliferation effect against both cisplatin sensitive and resistant human ovarian cell lines. Further research might result in discovery of agent(s) that can potentially be useful as an adjunct therapy for ovarian cancer cells. It is one of the few North American medicinal herbs that have been tested for anti-ovarian cancer activities.
为了寻找更有效的卵巢癌治疗方法，我们研究了北美重要药用植物魔鬼爪（OH）根皮提取物对顺铂敏感和耐药人卵巢癌细胞系的体外抗增殖活性。
高效液相色谱法用于提供 OH 提取物的化学分析。通过结晶紫测定法，单独或与顺铂或紫杉醇联合使用 OH 提取物对四种人卵巢癌细胞系的抗增殖活性进行了测定。流式细胞术和相差显微镜用于细胞周期分析，并检测细胞凋亡。
我们的数据表明，OH 的水、70%乙醇、100%乙醇和乙酸乙酯提取物在体外抑制了人卵巢癌细胞系 A2780、A2780CP70、OVCAR3 和 OVCAR10 的增殖。相应的 50%抑制浓度（IC50）估计为 1/256、1/74、1/69、1/53；1/4156、1/1847、1/1029、1/4530；1/25、753、1/3310、1/3462、1/5049；和 1/29、916、1/2912、1/3828 和 1/4232 稀释度。非细胞毒性稀释度（<IC50）的 70%乙醇 OH 提取物与顺铂和紫杉醇的一些组合增强了其对 A2780 和 A2780CP70 细胞的抗增殖 IC50。细胞周期分析表明，OH 提取物对细胞周期的影响取决于所测试的浓度，将细胞阻滞在 S 和 G2/M 期。在低浓度下，它通过细胞凋亡诱导细胞死亡，而在高浓度下，它通过坏死杀死细胞。
我们的数据表明，OH 提取物对顺铂敏感和耐药的人卵巢癌细胞系均表现出显著的抗增殖作用。进一步的研究可能会发现潜在的辅助治疗卵巢癌细胞的药物。它是少数经过测试具有抗卵巢癌活性的北美药用植物之一。
