Department of General and Social Psychiatry, Innsbruck Medical University, Austria.
Exp Gerontol. 2010 Jan;45(1):70-4. doi: 10.1016/j.exger.2009.10.005. Epub 2009 Oct 29.
Alzheimer's disease (AD) is a progressive neurodegenerative disorder with characteristic neuropathological features that are accompanied by inflammatory processes and release of pro-inflammatory cytokines. There is evidence that microglial cells are a key mediator of damage in AD. The microglial compartment may arise to a greater part from activation and transmigration of circulating monocytes. The aim of the present pilot study was to explore, if different cell adhesion molecules (ICAM-1 and -3, PECAM-1, VCAM-1, P-, L- and E-selectins, E-cadherin), RAGE and CD14 are affected in monocytes of healthy subjects compared to patients suffering from AD or mild cognitive impairment (MCI). Monocytes were isolated by negative magnetic selection (MACS) from EDTA blood samples, and extracts were analyzed by Searchlight Multiplex ELISAs. When compared to healthy subjects, the ratio of monocytic ICAM-3/CD14 was significantly decreased in MCI and AD patients and the ratio of the monocytic P-selectin/CD14 was specifically decreased in AD patients. In conclusion, our data show that monocytic cell adhesion molecules are decreased in AD and MCI patients. Further larger longitudinal studies should then clarify whether any of these parameters may be useful as a diagnostic biomarker.
阿尔茨海默病（AD）是一种进行性神经退行性疾病，具有特征性的神经病理学特征，伴有炎症过程和促炎细胞因子的释放。有证据表明，小胶质细胞是 AD 损伤的关键介质。小胶质细胞区室可能更多地来源于循环单核细胞的激活和迁移。本初步研究旨在探讨与健康受试者相比，患有 AD 或轻度认知障碍（MCI）的患者的单核细胞中是否存在不同的细胞粘附分子（ICAM-1 和 -3、PECAM-1、VCAM-1、P-、L- 和 E-选择素、E-钙黏蛋白）、RAGE 和 CD14 的变化。使用阴性磁选（MACS）从 EDTA 血液样本中分离单核细胞，并通过 Searchlight 多重 ELISA 进行分析。与健康受试者相比，MCI 和 AD 患者的单核细胞 ICAM-3/CD14 比值显著降低，AD 患者的单核细胞 P-选择素/CD14 比值特异性降低。总之，我们的数据表明 AD 和 MCI 患者的单核细胞粘附分子减少。然后应进行更大的纵向研究，以阐明这些参数中的任何一个是否可作为有用的诊断生物标志物。
