Erl W, Weber C, Wardemann C, Weber P C
Institut für Prophylaxe und Epidemiologie der Kreislaufkrankheiten, Universität München, Germany.
Atherosclerosis. 1995 Feb;113(1):99-107. doi: 10.1016/0021-9150(94)05434-k.
Progress in the understanding of blood cell--endothelial cell interactions has been achieved by the development of in-vitro model systems. We describe adhesion properties of the recently established human monocytic cell line Mono Mac 6. These cells showed increased adherence to unstimulated and tumour necrosis factor (TNF)-alpha (50 U/ml) stimulated human umbilical vein endothelial cells (HUVEC) (9.4% +/- 0.4% and 56.5% +/- 3.3%), as compared to U937 cells (2.6% +/- 0.8% and 40.0% +/- 8.4%). The values were similar to freshly isolated human blood monocytes (18.8% +/- 7.5% and 55.7% +/- 9.3%, respectively). Maximal binding was 6.2 +/- 0.6 Mono Mac 6 cells per HUVEC, which was 34% less than U937 cells (8.9 +/- 0.3). The lower number of adherent Mono Mac 6 cells per HUVEC could be due to their larger size, as assessed by flow cytometry. Blocking experiments with monoclonal antibody (mAb) directed against E-selectin, VCAM-1 and ICAM-1 on HUVEC and CD11b or CD14 on Mono Mac 6 cells demonstrated the contribution of these molecules to Mono Mac 6 adherence. Reduced binding after 24 h parallels the decline of E-selectin expression in HUVEC. Linearity of cell binding was confirmed from 0.2 x 10(6) to 1.0 x 10(6) Mono Mac 6 cells. Expression of CD11b and CD14 in Mono Mac 6 cells and in isolated human monocytes but not in U937 cells leading to interaction with ICAM-1 on HUVEC appears to be responsible for the increased adhesion of Mono Mac 6, as compared to U937 cells.(ABSTRACT TRUNCATED AT 250 WORDS)
通过体外模型系统的发展，人们对血细胞与内皮细胞相互作用的理解取得了进展。我们描述了最近建立的人单核细胞系Mono Mac 6的黏附特性。与U937细胞（分别为2.6%±0.8%和40.0%±8.4%）相比，这些细胞对未刺激的和肿瘤坏死因子（TNF）-α（50 U/ml）刺激的人脐静脉内皮细胞（HUVEC）的黏附增加（分别为9.4%±0.4%和56.5%±3.3%）。这些数值与新鲜分离的人血单核细胞相似（分别为18.8%±7.5%和55.7%±9.3%）。每个HUVEC上最大结合的Mono Mac 6细胞数为6.2±0.6，比U937细胞少34%（8.9±0.3）。通过流式细胞术评估，每个HUVEC上黏附的Mono Mac 6细胞数量较少可能是由于其较大的尺寸。用针对HUVEC上E-选择素、VCAM-1和ICAM-1以及Mono Mac 6细胞上CD11b或CD14的单克隆抗体（mAb）进行的阻断实验证明了这些分子对Mono Mac 6黏附的作用。24小时后结合减少与HUVEC中E-选择素表达的下降平行。从0.2×10⁶到1.0×10⁶个Mono Mac 6细胞，细胞结合呈线性关系得到证实。与U937细胞相比，Mono Mac 6细胞和分离的人单核细胞中CD11b和CD14的表达导致与HUVEC上ICAM-1相互作用，这似乎是Mono Mac 6黏附增加的原因。（摘要截断于250字）
