Department of Materials Science, Osaka Prefecture University, Japan.
Acta Biomater. 2010 Mar;6(3):830-5. doi: 10.1016/j.actbio.2009.10.019. Epub 2009 Oct 31.
Loading and releasing protein in a controllable way is extremely important for the protein vehicles used in bone tissue engineering. To obtain a suitable carrier material for basic proteins, such as BMP or bFGF, hydroxyapatite particles containing mercaptosuccinic acid (mercaptosuccinic acid (Mer), (Mer-HAp)) were synthesized. Physicochemical evaluation of Mer-HAp suggested that Mer was contained in HAp particles: it either simply adsorbed onto HAp crystals or was trapped among the HAp crystals. A protein adsorption study using basic and acidic model proteins indicated that the synthesized Mer-HAp had selective loading properties of the basic protein. The loaded protein was gradually released from Mer-HAp in phosphate buffered saline. The protein release rate was different in each Mer-HAp synthesized with a different concentration of Mer. In addition, the protein release from Mer-HAp showed a similar profile with the Ca dissolution in different pH solutions, indicating that the Mer-HAp dissolution was concerned with the protein release from Mer-HAp. Thus, Mer-HAp is a useful candidate for the basic protein carrier because it has properties which enable the loading and releasing of protein in a controllable way.
以可控的方式加载和释放蛋白质对于骨组织工程中使用的蛋白质载体非常重要。为了获得适合碱性蛋白质（如 BMP 或 bFGF）的载体材料，合成了含有巯基琥珀酸（mercaptosuccinic acid (Mer), (Mer-HAp)）的羟基磷灰石颗粒。对 Mer-HAp 的物理化学评价表明，Mer 存在于 HAp 颗粒中：它要么简单地吸附在 HAp 晶体上，要么被困在 HAp 晶体之间。使用碱性和酸性模型蛋白质进行的蛋白质吸附研究表明，合成的 Mer-HAp 对碱性蛋白质具有选择性的负载特性。负载的蛋白质在磷酸盐缓冲盐水中逐渐从 Mer-HAp 中释放出来。用不同浓度的 Mer 合成的 Mer-HAp 的蛋白质释放速率不同。此外，Mer-HAp 中的蛋白质释放与不同 pH 值溶液中 Ca 的溶解具有相似的模式，表明 Mer-HAp 的溶解与 Mer-HAp 中蛋白质的释放有关。因此，Mer-HAp 是一种有用的碱性蛋白质载体候选物，因为它具有以可控方式加载和释放蛋白质的特性。
