Department of Oromaxillofacial Regeneration, Osaka University, 1-8 Yamada-oka, Suita, 565-0871, Japan.
J Dent Res. 2010 May;89(5):488-92. doi: 10.1177/0022034509357309. Epub 2010 Feb 16.
Physico-chemical modifications of hydroxyapatite (HAp) materials are considered as pre-requisites for the development of new bioactive carrier materials for drug delivery and tissue engineering applications. Since acidic amino acids have well-documented affinities to both HAp and basic proteins, HAp modified by aspartic acid (Asp, acidic amino acid) might be one of the candidate substrates for a basic protein carrier. Here, we synthesized HAp in the presence of various concentrations of Asp and observed that HAp crystallinity and other physico-chemical properties were effectively modulated. Detailed studies indicated that Asp was not incorporated in the HAp crystal lattice, but rather was trapped in HAp crystals. Protein adsorption studies indicated that the HAp particles modified by Asp had a selective loading capacity for basic protein. Therefore, HAp particles containing Asp might have potential in drug delivery applications, especially as the carrier of basic proteins including bFGF and BMP.
对羟基磷灰石（HAp）材料进行物理化学修饰被认为是开发新的用于药物输送和组织工程应用的生物活性载体材料的前提条件。由于酸性氨基酸与 HAp 和碱性蛋白质都具有很好的亲和力，因此被天冬氨酸（Asp，酸性氨基酸）修饰的 HAp 可能是碱性蛋白质载体的候选底物之一。在这里，我们在存在各种浓度的 Asp 的情况下合成了 HAp，并观察到 HAp 的结晶度和其他物理化学性质得到了有效调节。详细研究表明，Asp 并未掺入 HAp 晶格中，而是被困在 HAp 晶体中。蛋白质吸附研究表明，经 Asp 修饰的 HAp 颗粒对碱性蛋白质具有选择性负载能力。因此，含有 Asp 的 HAp 颗粒可能在药物输送应用中具有潜力，特别是作为碱性蛋白质（包括 bFGF 和 BMP）的载体。
