Chemistry Institute, State University of Campinas, Campinas, SP, Brazil.
Talanta. 2009 Dec 15;80(2):428-33. doi: 10.1016/j.talanta.2009.06.080. Epub 2009 Jul 8.
An imaging mass spectrometric method using laser ablation inductively coupled plasma spectrometry (LA-ICP-MS) was developed to determine Cu, Zn, Cd, Hg and Pb and metal distribution in longitudinal tissue sections of the marine snail Nassarius reticulatus (Gastropoda, Prosobranchia). Snails were sampled in northern Brittany (France) at three stations with different contamination levels. The quantification of metal distribution (imaging or mapping) in a thin slice of the snail tissue was carried out using different strategies: by one-point calibration and via matrix-matched laboratory standards using different biological materials (BCR 278, snail tissue, and rat brain). Together with the imaging of metals the distribution of two non-metals (carbon and sulfur) was analyzed. The imaging LA-ICP-MS analysis yielded an inhomogeneous distribution for all elements investigated. The detection limits for the distribution analysis of Cu, Zn, Cd, Hg and Pb measured by LA-ICP-MS were in the low microg g(-1) range.
建立了一种利用激光烧蚀电感耦合等离子体质谱法（LA-ICP-MS）的成像质谱方法，用于测定海洋蜗牛 Nassarius reticulatus（腹足纲，前鳃亚纲）纵向组织切片中的 Cu、Zn、Cd、Hg 和 Pb 以及金属分布。在法国布列塔尼北部的三个具有不同污染水平的站点采集了蜗牛。使用不同的策略对蜗牛组织薄片中的金属分布（成像或映射）进行定量：通过单点校准和使用不同生物材料（BCR 278、蜗牛组织和大鼠脑）的基质匹配实验室标准。除了金属成像外，还分析了两种非金属（碳和硫）的分布。成像 LA-ICP-MS 分析得到了所有研究元素的不均匀分布。通过 LA-ICP-MS 测量，Cu、Zn、Cd、Hg 和 Pb 分布分析的检测限处于低微 g/g(-1) 范围内。
