State Key Laboratory of Biogeology and Environmental Geology, School of Earth Sciences, China University of Geosciences, Wuhan, 430074, PR China.
Hangzhou PuYu Technology Development Co., Ltd., Hangzhou, 311300, PR China.
Anal Bioanal Chem. 2024 Aug;416(20):4519-4529. doi: 10.1007/s00216-024-05391-w. Epub 2024 Jun 16.
A simple and reliable external calibration strategy of LA-ICP-MS for fresh plant soft tissues was developed. The prepared plant suspension was frozen by the designed cryogenic ablation cell and used as external standard for quantitative elemental imaging analysis of fresh plant tissues. The controllable water content of the prepared external standards provides a similar matrix with fresh soft tissues, and a homogeneous elemental distribution could be ensured due to the fine grinding particle sizes. More interestingly, the presence of water increased the signal intensity produced by the suspension by a factor of 1.6 (Pb) to 66.6 (La) compared to that of the pressed cake. The excellent dispersing property and advantage of long-term use were achieved owing to the employment of 0.1% PAANa as suspending agent. A series of plant reference materials were analyzed, and the relative errors of most elements were less than 10 %, indicating that there is a reliable accuracy of the proposed method. The limits of detection (LODs) ranged from 0.1 ng·g (La) to 1279 ng·g (S). This method was used for elemental imaging analysis in rice leaves under arsenic stress, and the results were consistent with previous studies, which mean that the proposed method could provide technical support for researchers in the fields of agriculture and environment.
本研究建立了一种简单可靠的用于新鲜植物软组织的激光剥蚀电感耦合等离子体质谱(LA-ICP-MS)外部校准策略。将制备的植物悬浮液用设计的低温消融池冷冻,作为新鲜植物组织定量元素成像分析的外部标准。所制备的外部标准的可控水含量提供了与新鲜软组织相似的基质,并且由于细磨粒径可以确保均匀的元素分布。更有趣的是,与压饼相比,悬浮液中的水使信号强度增加了 1.6 倍(Pb)至 66.6 倍(La)。由于使用了 0.1% PAANa 作为悬浮剂,实现了良好的分散性和长期使用的优势。分析了一系列植物标准物质,大多数元素的相对误差均小于 10%,表明该方法具有可靠的准确性。检测限(LOD)范围为 0.1ng·g(La)至 1279ng·g(S)。该方法用于砷胁迫下水稻叶片的元素成像分析,结果与先前的研究一致,这意味着该方法可为农业和环境领域的研究人员提供技术支持。