急性和慢性根尖周脓肿中明胶酶（基质金属蛋白酶-2和-9）水平的酶谱分析
The zymographic evaluation of gelatinase (MMP-2 and -9) levels in acute and chronic periapical abscesses.
作者信息
Buzoglu Hatice Dogan, Unal Hasan, Ulger Celal, Mert Safak, Kücükyildirim Sibel, Er Nuray
机构信息
Faculty of Dentistry, Department of Endodontics, Hacettepe University, Ankara, Turkey.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Nov;108(5):e121-6. doi: 10.1016/j.tripleo.2009.07.014.
OBJECTIVE
This study investigated the presence and levels of matrix metalloproteinases (MMP)-2 and -9 in periapical abscesses.
STUDY DESIGN
Eighteen samples of intracanal exudates containing pus were collected from teeth with clinically and radiographically verified primary or secondary acute and chronic apical abscesses. Pro- and active forms of MMP-2 and MMP-9 levels were analyzed by using substrate gel zymography followed by an image analysis system. Statistical analysis was performed using the Kruskall-Wallis and Mann-Whitney U tests with Bonferroni adjustment.
RESULTS
Both forms of MMP-9 were detected in all pus samples and demonstrated marked differences among the experimental groups (P < .05). Primary or secondary acute apical abscess samples demonstrated significantly higher MMP-9 levels compared with MMP-2 levels (P < .01). However, MMP-2 could not be detected in chronic apical abscesses.
CONCLUSION
According to the results of this study, gelatinases might affect the pathogenesis of acute and chronic periapical abscesses.
目的
本研究调查根尖周脓肿中基质金属蛋白酶（MMP）-2和-9的存在情况及水平。
研究设计
从临床和影像学检查证实为原发性或继发性急慢性根尖脓肿的牙齿中收集18份含有脓液的根管内渗出物样本。采用底物凝胶酶谱法结合图像分析系统分析MMP-2和MMP-9的前体形式和活性形式水平。使用经Bonferroni校正的Kruskall-Wallis检验和Mann-Whitney U检验进行统计分析。
结果
在所有脓液样本中均检测到两种形式的MMP-9，且各实验组之间存在显著差异（P <.05）。原发性或继发性急性根尖脓肿样本中MMP-9水平显著高于MMP-2水平（P <.01）。然而，在慢性根尖脓肿中未检测到MMP-2。
结论
根据本研究结果，明胶酶可能影响急慢性根尖周脓肿的发病机制。
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