Shin Su-Jung, Lee Jae-Il, Baek Seung-Ho, Lim Sung-Sam
Department of Conservative Dentistry, College of Dentistry, Seoul National University, Korea.
J Endod. 2002 Apr;28(4):313-5. doi: 10.1097/00004770-200204000-00013.
The purpose of this study was to evaluate the tissue levels of matrix metalloproteinase (MMP)-1, -2, -3 and their distributions in inflamed human dental pulps and periapical lesions. Samples were subjected to enzyme-linked immunosorbent assay and/or immunohistochemistry by using specific antibodies to MMP-1, -2, and -3. Results from enzyme-linked immunosorbent assay were analyzed by using the Mann-Whitney U test and presented as p values. The concentrations of MMP-1 in all experimental groups were significantly higher than in the control (p < 0.05). The acute pulpitis and control groups were significant different in terms of their MMP-2 levels (p < 0.05). The concentration of MMP-3 in acute pulpitis was significantly higher than the control and chronic pulpitis groups (p < 0.05). Immunohistochemically, MMP-1 and MMP-3 were localized in the infiltrating neutrophils, macrophages, and extracellular matrix of the acute pulpitis group. These results suggest that MMPs play an important role in the pulp tissue destruction of acute, inflamed pulp.
本研究的目的是评估基质金属蛋白酶（MMP）-1、-2、-3在人牙髓炎症和根尖周病变中的组织水平及其分布情况。通过使用针对MMP-1、-2和-3的特异性抗体，对样本进行酶联免疫吸附测定和/或免疫组织化学分析。酶联免疫吸附测定的结果采用Mann-Whitney U检验进行分析，并以p值表示。所有实验组中MMP-1的浓度均显著高于对照组（p < 0.05）。急性牙髓炎组和对照组的MMP-2水平存在显著差异（p < 0.05）。急性牙髓炎组中MMP-3的浓度显著高于对照组和慢性牙髓炎组（p < 0.05）。免疫组织化学结果显示，MMP-1和MMP-3定位于急性牙髓炎组的浸润性中性粒细胞、巨噬细胞和细胞外基质中。这些结果表明，基质金属蛋白酶在急性炎症牙髓的牙髓组织破坏中起重要作用。
