从牙髓细胞获得的条件培养基的分化诱导作用。
The differentiation-inducing effect of conditioned media obtained from dental pulp cells.
作者信息
Kim Na Ryoung, Lee Dong Hee, Ahn Sug-Joon, Lee In-Seop, Yang Hyeong-Cheol
机构信息
Department of Dental Biomaterials Science, School of Dentistry, Seoul National University, Seoul, Korea.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 May;107(5):e54-9. doi: 10.1016/j.tripleo.2009.01.047.
OBJECTIVE
Conditioned media (CM) from human dental pulp cells (HDPC) was investigated for its effects on the proliferation and differentiation of HDPC and MG63 cells.
STUDY DESIGN
CM prepared from the primary culture of HDPC was used for the culture of HDPC and MG63. Cell growth, alkaline phosphatase (ALPase) activity, mRNA expression of differentiation-related genes, and mineralization of the HDPC and MG63 cells in the media containing CM were assessed.
RESULTS
HDPC CM increased the cell growth of HDPC but decreased MG63 cell growth. ALPase activity and the mineralization of both HDPC and MG63 were enhanced by HDPC CM. The CM also up-regulated the expressions of DSPP, DMP-1, and OCN mRNA in HDPC. Pretreatment of HDPC CM with a neutralizing antibody against TGF-beta completely eliminated the effect of CM on ALPase activity in HDPC.
CONCLUSION
HDPC was able to secrete odontogenic differentiation-inducing factors, in which TGF-beta seems to a key element of the CM effects.
目的
研究人牙髓细胞（HDPC）的条件培养基（CM）对HDPC和MG63细胞增殖及分化的影响。
研究设计
用HDPC原代培养制备的CM培养HDPC和MG63。评估含CM的培养基中HDPC和MG63细胞的生长、碱性磷酸酶（ALPase）活性、分化相关基因的mRNA表达及矿化情况。
结果
HDPC CM促进HDPC细胞生长，但抑制MG63细胞生长。HDPC CM增强了HDPC和MG63的ALPase活性及矿化能力。CM还上调了HDPC中DSPP、DMP-1和OCN mRNA的表达。用抗TGF-β中和抗体预处理HDPC CM可完全消除CM对HDPC中ALPase活性的影响。
结论
HDPC能够分泌牙源性分化诱导因子，其中TGF-β似乎是CM发挥作用的关键因素。
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