College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
Vaccine. 2009 Dec 11;28(2):523-31. doi: 10.1016/j.vaccine.2009.09.130. Epub 2009 Nov 3.
A swine influenza virus (SIV) vaccine-challenge pig model was used to study the potential of a conserved matrix 2 (M2) protein vaccine alone or in combination with an inactivated H1N1-vaccine to protect against H1N1 and H1N2 viruses. The H1N1-vaccine and heterologous H1N2-challenge virus model has previously been shown to prolong fever and increase SIV-associated pneumonic lesions. The M2 vaccine in combination with the H1N1-vaccine reduced the H1N2 induced fever but not virus shedding. The M2 vaccine alone reduced respiratory signs and pneumonic lesions to levels similar to the negative control pigs following H1N2 infection. This study found that the M2 protein has potential as a vaccine for SIV-associated disease prevention. However, development of an immune response towards the major envelope HA protein was required to reduce SIV shedding.
猪流感病毒（SIV）疫苗攻毒猪模型被用于研究单独使用或与灭活 H1N1 疫苗联合使用保守基质 2（M2）蛋白疫苗预防 H1N1 和 H1N2 病毒的潜力。先前的研究表明，H1N1 疫苗和异源 H1N2 攻毒病毒模型可延长发热时间并增加 SIV 相关的肺炎病变。M2 疫苗与 H1N1 疫苗联合使用可降低 H1N2 引起的发热，但不能减少病毒脱落。单独使用 M2 疫苗可降低呼吸道症状和肺炎病变，使其在感染 H1N2 后与阴性对照猪相似。本研究发现，M2 蛋白具有作为 SIV 相关疾病预防疫苗的潜力。然而，需要针对主要包膜 HA 蛋白产生免疫反应以减少 SIV 脱落。
