Liu Ying, Feng Jin-Chao, Li Dan, Cui Jian, Xu Si-Fan, Shen Gang-Yi
College of Life and Environmental Science, Minzu University of China, Beijing 100081, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Aug;29(8):2217-21.
Fluorescence microscopic self-ordered ring (SOR) technique with microwave heating is proposed for minocycline based on the capillary flow of solvent on the surface of hydrophobic glass slide and applied to residues detection of the antibiotics in milks of Inner Mongolia. In the reaction medium of hexahydropyridine containing polyvinyl alcohol-124 (PVA-124), a SOR of minocycline with the outer diameter of 1.54 mm and the ring belt width of 22.6 microm can be formed. When a 0.30 microL droplet of minocycline mixture was spotted on the solid surface, minocycline in the range of 4.2 x 10(-2)-1.8 x 10(-11) mol x ring(-1) (1.4 x 10(-6)-0.60 x 10(-5) mol x L(-1)) can be detected, the maximum fluorescence intensity was found to be proportional to the minocycline concentration, and the limit of detection can reach 4.2 x 10(-13) mol x ring(-1) (1.4 x 10(-7) mol x L(-1)) with three times of signal to noise ratio. With the present method, the contents of minocycline in milk samples of Inner Mongolia and minocycline hydrochloride capsule sample were satisfactorily determined with recoveries of 97.2%-103% and 99.4%-102%, respectively, and RSD lower than 1.2%, correspondingly. Therefore, a highly sensitive and selective analysis method for detecting content of trace drug contaminations was established. The method provided theory basis to quantificational analysis of the residues of the antibiotics in milks which was Inner Mongolia' preponderant resource products. It has important realistic meaning and broad application prospect.
基于疏水性载玻片表面溶剂的毛细流动，提出了一种采用微波加热的荧光显微镜自组装环（SOR）技术用于米诺环素检测，并将其应用于内蒙古牛奶中抗生素残留的检测。在含有聚乙烯醇-124（PVA-124）的六氢吡啶反应介质中，可形成外径为1.54 mm、环带宽度为22.6微米的米诺环素自组装环。当将0.30微升的米诺环素混合物液滴点样在固体表面时，可检测到4.2×10⁻² - 1.8×10⁻¹¹摩尔/环（1.4×10⁻⁶ - 0.60×10⁻⁵摩尔/升）范围内的米诺环素，最大荧光强度与米诺环素浓度成正比，在信噪比为三倍时，检测限可达4.2×10⁻¹³摩尔/环（1.4×10⁻⁷摩尔/升）。采用该方法，内蒙古牛奶样品和盐酸米诺环素胶囊样品中米诺环素的含量得到了满意的测定，回收率分别为97.2% - 103%和99.4% - 102%，相对标准偏差相应低于1.2%。因此，建立了一种高灵敏度和高选择性的痕量药物污染物含量检测分析方法。该方法为内蒙古优势资源产品牛奶中抗生素残留的定量分析提供了理论依据。具有重要的现实意义和广阔的应用前景。
