Huang Cheng Zhi, Liu Ying, Li Yuan Fang
Laboratory of Supramolecular Chemistry and Biomedical Analysis, Chemistry and Chemical Engineering College, Southwest Normal University, Chongqing 400715, PR China.
J Pharm Biomed Anal. 2004 Jan 27;34(1):103-14. doi: 10.1016/j.japna.2003.08.006.
A fluorescent microscopic determination of trace amount of tetracycline is reported based on the aluminum-sensitized fluorescence effect of a self-ordered ring formed by a sessile droplet on glass slide support. Since the evaporative loss of the solvent from the edge wedge of the droplet that is spotted on a hydrophobic-treated glass slide, an outward capillary flow of the interior solvent of the droplet occurs. The resulted outward capillary flow then carries the solute to the perimeter of the droplet spot where the solute accumulates to form a fluorescent self-ordered ring (SOR). Depending on the spotted volume of the aluminum-tetracycline chelate solution, different size of SOR with the outer diameter (o.d.) less than 1.1mm and the ring belt width less than 21.6 microm can be obtained. Data analysis for the imaged SOR by using a digitalized CCD camera showed that the chelate molecule across the fluorescent SOR belt section follows a Gaussian distribution, and the maximum fluorescent intensity (I(max)) was found to be proportional to tetracycline content. When a 0.1 microl droplet was spotted on the solid surface, tetracycline in the range of 7.5-800.0 fmol (or 7.9 x 10(-8) to 800.0 x 10(-8)moll (-1)) can be detected, and the limit of detection can reach 0.8 fmol (or 7.9 x 10(-9) moll (-1)). With present method, the contents of tetracycline in capsule, tablet, urine and fresh milk were satisfactorily detected with the recoveries of 97.0-106.5% and RSD of 1.2-4.2%, correspondingly.
报道了一种基于玻片上固着液滴形成的自组装环的铝敏化荧光效应来测定痕量四环素的荧光显微镜方法。由于点样在经疏水处理的玻片上的液滴边缘楔形区域溶剂的蒸发损失，液滴内部溶剂会发生向外的毛细管流动。由此产生的向外毛细管流动将溶质带到液滴斑点的周边，溶质在那里积累形成荧光自组装环（SOR）。根据铝 - 四环素螯合物溶液的点样体积，可以获得外径小于1.1mm且环带宽度小于21.6微米的不同尺寸的SOR。使用数字化电荷耦合器件（CCD）相机对成像的SOR进行数据分析表明，穿过荧光SOR带截面的螯合分子呈高斯分布，并且发现最大荧光强度（I(max)）与四环素含量成正比。当在固体表面点样0.1微升液滴时，可以检测到7.5 - 800.0飞摩尔（或7.9×10^(-8)至800.0×10^(-8)摩尔/升）范围内的四环素，检测限可达0.8飞摩尔（或7.9×10^(-9)摩尔/升）。采用本方法，对胶囊、片剂、尿液和新鲜牛奶中的四环素含量进行了满意的检测，回收率为97.0 - 106.5%，相对标准偏差为1.2 - 4.2%。
