Lamponi Stefania, Di Canio Clara, Barbucci Rolando
Department of Pure and Applied Medicinal Chemistry, University of Siena, Siena, Italy.
Int J Artif Organs. 2009 Aug;32(8):507-16. doi: 10.1177/039139880903200805.
The aim of this paper was to study the influence of chemical and topographical signals on cell behavior and to obtain a heterotypic cell-cell interaction on microstructured domains.
The polysaccharide hyaluronic acid (Hyal) was photoimmobilized on glass surfaces in order to obtain a pattern with squares and rectangles of different dimensions and chemistry. The microstructured surfaces were characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The behavior of human coronary artery endothelial cells (HCAEC) and human tumoral dermal fibroblasts (C54) was investigated on these micropatterned surfaces by adhesion studies. Moreover heterotypic interaction among C54 and HCAEC adherent on patterned surfaces was evaluated by time-lapse video microscopy
Surface analysis revealed the presence of a pattern consisting of alternating glass and Hyal microstructures whose dimensions decreased from the center to the edge of the sample. Neither HCAEC nor C54 adhered to the immobilized Hyal but both adapted their shape to the different sizes of the glass squares and rectangles. The number of adherent cells depended on the dimensions of both the glass domains and the nuclei of the cells. Co-cultured C54 on HCAEC patterned surfaces showed a heterotypic cell-cell interaction in the same chemical and topographic domain.
A heterotypic cell-cell interaction occurred in the same chemical and topographic micro-domains but in narrow areas only. Moreover, the number of cells adhering to the glass domains and cell morphology depended on the dimensions of both adhesive areas and cell nuclei.
本文旨在研究化学信号和拓扑信号对细胞行为的影响，并在微结构化区域获得异型细胞间相互作用。
将多糖透明质酸（Hyal）光固定在玻璃表面，以获得具有不同尺寸和化学性质的正方形和矩形图案。通过扫描电子显微镜（SEM）和原子力显微镜（AFM）对微结构化表面进行表征。通过黏附研究，在这些微图案化表面上研究人冠状动脉内皮细胞（HCAEC）和人肿瘤真皮成纤维细胞（C54）的行为。此外，通过延时视频显微镜评估黏附在图案化表面上的C54和HCAEC之间的异型相互作用。
表面分析显示存在由交替的玻璃和Hyal微结构组成的图案，其尺寸从样品中心到边缘逐渐减小。HCAEC和C54均未黏附于固定化的Hyal，但两者均使其形状适应玻璃正方形和矩形的不同尺寸。黏附细胞的数量取决于玻璃区域的尺寸和细胞核的大小。在HCAEC图案化表面上共培养的C54在相同的化学和拓扑区域显示出异型细胞间相互作用。
异型细胞间相互作用发生在相同的化学和拓扑微区域，但仅在狭窄区域。此外，黏附在玻璃区域的细胞数量和细胞形态取决于黏附区域和细胞核的尺寸。
