Lamponi Stefania, Forbicioni Marco, Barbucci Rolando
Department of Pure and Applied Medicinal Chemistry and C.R.I.S.M.A, University of Siena, Siena - Italy.
J Appl Biomater Biomech. 2009 May-Aug;7(2):104-10.
Spiral micropatterned surfaces of decreasing dimensions were produced by photo-immobilizing a photo-reactive hyaluronan (Hyal) derivative on TiO2 nanoparticles. The microstructured surfaces were characterized by both scanning electron microscopy and atomic force microscopy analysis. The behavior, of both endothelial cells (HCAEC) and tumoral mouse fibroblasts (NIH3T3) on the patterned surfaces was evaluated. HCAEC adhered only to the TiO2 nanoparticles avoiding contact with the Hyal. NIH3T3 adhered to and completely covered the TiO2 spiral but prolonging the culture time, it also covered the external photo-immobilized Hyal surface. The role of fibronectin to mediate cell adhesion to the TiO2 pattern surfaces was evaluated by experiments with blocked fibronectin membrane receptors on both HCAEC and NIH3T3. The results showed the absence of any adhering cells. Therefore, fibronectin seemed to be the only key protein in mediating cell adhesion to these TiO2 substrates.
通过将光反应性透明质酸（Hyal）衍生物光固定在二氧化钛纳米颗粒上，制备出尺寸递减的螺旋微图案表面。通过扫描电子显微镜和原子力显微镜分析对微结构表面进行了表征。评估了内皮细胞（HCAEC）和肿瘤小鼠成纤维细胞（NIH3T3）在图案化表面上的行为。HCAEC仅附着于二氧化钛纳米颗粒，避免与透明质酸接触。NIH3T3附着并完全覆盖了二氧化钛螺旋，但随着培养时间的延长，它也覆盖了外部光固定的透明质酸表面。通过对HCAEC和NIH3T3上的纤连蛋白膜受体进行阻断实验，评估了纤连蛋白介导细胞黏附到二氧化钛图案表面的作用。结果显示没有任何黏附细胞。因此，纤连蛋白似乎是介导细胞黏附到这些二氧化钛底物的唯一关键蛋白。
