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碳源和氮源对担子菌胞外多糖产生的影响。

Carbon and nitrogen source effects on basidiomycetes exopolysaccharide production.

作者信息

Elisashvili V I, Kachlishvili E T, Wasser S P

机构信息

Durmishidze Institute of Biochemistry and Biotechnology, 10 km Agmashenebeli alley, 0159 Tbilisi, Georgia.

出版信息

Prikl Biokhim Mikrobiol. 2009 Sep-Oct;45(5):592-6.

PMID:19845293
Abstract

The capability to synthesize the extracellular polysaccharide (EPS) is widespread among eight mushroom species which accumulated 0.6-2.2 g/l of EPS in submerged cultivation. Glucose, maltose, and mannitol were the most appropriate carbon sources for biomass and EPS production. Organic nitrogen sources appeared to be the most suitable nitrogen sources for biomass and EPS accumulation. The cultivation process in shake flasks was successfully reproduced in a laboratory fermentor with enhanced EPS production. The highest yield of EPS (3.8-4.0 g/l) was achieved in cultivation of Agaricus nevoi and Inonotus levis.

摘要

合成细胞外多糖(EPS)的能力在八种蘑菇物种中广泛存在,这些蘑菇在深层培养中积累了0.6 - 2.2克/升的EPS。葡萄糖、麦芽糖和甘露醇是生物质和EPS生产最合适的碳源。有机氮源似乎是生物质和EPS积累最合适的氮源。摇瓶培养过程在实验室发酵罐中成功重现,且EPS产量有所提高。在培养阿氏蘑菇和轻毛栓菌时,EPS产量最高(3.8 - 4.0克/升)。

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