Lee Sang-Hoon, Senevirathne Mahinda, Ahn Chang-Bum, Kim Se-Kwon, Je Jae-Young
Department of Chemistry, Pukyong National University, Busan 606-737, Republic of Korea.
Bioorg Med Chem Lett. 2009 Dec 1;19(23):6655-8. doi: 10.1016/j.bmcl.2009.10.007. Epub 2009 Oct 7.
In this study, factors affecting anti-inflammatory effect of chitooligosaccharides (COSs) in lipopolysaccharides (LPS)-induced RAW264.7 macrophage cells were investigated. The inhibition of NO secretion by COSs revealed that 90-COSs (90% N-deacetylation) significantly inhibited NO secretion than those of 50-COSs (50% N-deacetylation), and 90-HMWCOS (5000-10,000Da) in the 90-COSs showed the highest inhibition activity. Furthermore, 90-HMWCOSs also found to inhibit LPS-stimulated production of PGE(2), TNF-alpha and IL-6, as well as the expression of iNOS, COX-2, TNF-alpha, and IL-6. These results suggested that 90-HMWCOS may have anti-inflammatory effect via down-regulation of transcriptional and translational expression levels of TNF-alpha, IL-6 and iNOS and COX-2.
在本研究中，对壳寡糖（COSs）在脂多糖（LPS）诱导的RAW264.7巨噬细胞中的抗炎作用影响因素进行了研究。COSs对NO分泌的抑制作用表明，90-COSs（90%N-脱乙酰化）比50-COSs（50%N-脱乙酰化）能更显著地抑制NO分泌，且90-COSs中的90-HMWCOS（5000-10000Da）表现出最高的抑制活性。此外，还发现90-HMWCOSs能抑制LPS刺激的PGE(2)、TNF-α和IL-6的产生，以及iNOS、COX-2、TNF-α和IL-6的表达。这些结果表明，90-HMWCOS可能通过下调TNF-α、IL-6、iNOS和COX-2的转录和翻译表达水平而具有抗炎作用。
