• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

N-乙酰化对壳寡糖抗炎活性的影响及其对内毒素血症的缓解作用。

The Effect of N-Acetylation on the Anti-Inflammatory Activity of Chitooligosaccharides and Its Potential for Relieving Endotoxemia.

机构信息

CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.

Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), No. 1 Wenhai Road, Qingdao 266237, China.

出版信息

Int J Mol Sci. 2022 Jul 26;23(15):8205. doi: 10.3390/ijms23158205.

DOI:10.3390/ijms23158205
PMID:35897781
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9330575/
Abstract

Endotoxemia is mainly caused by a massive burst of inflammatory cytokines as a result of lipopolysaccharide (LPS) invasion. Chitooligosaccharides (COS) is expected to be a potential drug for relieving endotoxemia due to its anti-inflammatory properties. However, the structural parameters of COS are often ambiguous, and the effect of degree of acetylation (DA) of COS on its anti-inflammatory remains unknown. In this study, four COSs with different DAs (0%, 12%, 50% and 85%) and the same oligomers distribution were successfully obtained. Their structures were confirmed by H NMR and MS analysis. Then, the effect of DA on the anti-inflammatory activity and relieving endotoxemia potential of COS was researched. The results revealed that COS with a DA of 12% had better anti-inflammatory activity than COSs with other DAs, mainly in inhibiting LPS-induced inflammatory cytokines burst, down-regulating its mRNA expression and reducing phosphorylation of IκBα. Furthermore, this COS showed an obviously protective effect on endotoxemia mice, such as inhibiting the increase in inflammatory cytokines and transaminases, alleviating the injury of liver and intestinal tissue. This study explored the effect of DA on the anti-inflammatory activity of COS for the first time and lays the foundation for the development of COS as an anti-inflammatory drug against endotoxemia.

摘要

内毒素血症主要是由于脂多糖 (LPS) 入侵导致炎症细胞因子大量爆发而引起的。壳寡糖 (COS) 因其具有抗炎特性，有望成为缓解内毒素血症的潜在药物。然而，COS 的结构参数通常不明确，并且 COS 的乙酰化程度 (DA) 对其抗炎作用的影响尚不清楚。在这项研究中，成功获得了具有不同 DA（0%、12%、50%和 85%）但具有相同低聚物分布的四种 COS。它们的结构通过 H NMR 和 MS 分析得到确认。然后，研究了 DA 对 COS 抗炎活性和缓解内毒素血症潜力的影响。结果表明，DA 为 12%的 COS 具有比其他 DA 更好的抗炎活性，主要表现在抑制 LPS 诱导的炎症细胞因子爆发、下调其 mRNA 表达和减少 IκBα 的磷酸化。此外，这种 COS 对内毒素血症小鼠表现出明显的保护作用，例如抑制炎症细胞因子和转氨酶的增加，减轻肝和肠组织的损伤。本研究首次探讨了 DA 对 COS 抗炎活性的影响，为开发 COS 作为治疗内毒素血症的抗炎药物奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d76/9330575/63369c30d45a/ijms-23-08205-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d76/9330575/7cbb9fd55614/ijms-23-08205-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d76/9330575/2f5d73168e19/ijms-23-08205-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d76/9330575/fc090877a851/ijms-23-08205-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d76/9330575/98e8f2851803/ijms-23-08205-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d76/9330575/17f70af6d536/ijms-23-08205-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d76/9330575/6e4a4cbe31ad/ijms-23-08205-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d76/9330575/28e7f1942b10/ijms-23-08205-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d76/9330575/c35cad82d42a/ijms-23-08205-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d76/9330575/be643b04fa2f/ijms-23-08205-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d76/9330575/63369c30d45a/ijms-23-08205-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d76/9330575/7cbb9fd55614/ijms-23-08205-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d76/9330575/2f5d73168e19/ijms-23-08205-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d76/9330575/fc090877a851/ijms-23-08205-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d76/9330575/98e8f2851803/ijms-23-08205-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d76/9330575/17f70af6d536/ijms-23-08205-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d76/9330575/6e4a4cbe31ad/ijms-23-08205-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d76/9330575/28e7f1942b10/ijms-23-08205-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d76/9330575/c35cad82d42a/ijms-23-08205-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d76/9330575/be643b04fa2f/ijms-23-08205-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d76/9330575/63369c30d45a/ijms-23-08205-g010.jpg

相似文献

1
The Effect of N-Acetylation on the Anti-Inflammatory Activity of Chitooligosaccharides and Its Potential for Relieving Endotoxemia.N-乙酰化对壳寡糖抗炎活性的影响及其对内毒素血症的缓解作用。
Int J Mol Sci. 2022 Jul 26;23(15):8205. doi: 10.3390/ijms23158205.
2
Impact of O-acetylation on chitin oligosaccharides modulating inflammatory responses in LPS-induced RAW264.7 cells and mice.O-乙酰化对几丁质寡糖调节脂多糖诱导的RAW264.7细胞和小鼠炎症反应的影响。
Carbohydr Res. 2024 Aug;542:109177. doi: 10.1016/j.carres.2024.109177. Epub 2024 Jun 6.
3
Green-Chemical Strategies for Production of Tailor-Made Chitooligosaccharides with Enhanced Biological Activities.绿色化学策略用于生产具有增强生物活性的定制壳寡糖。
Molecules. 2023 Sep 13;28(18):6591. doi: 10.3390/molecules28186591.
4
The water extract of "Jiao Mei Gu" attenuates the lipopolysaccharide-induced inflammatory response via inhibiting NF-κB activity in mice.“交莓果”水提取物通过抑制 NF-κB 活性减轻脂多糖诱导的小鼠炎症反应。
J Ethnopharmacol. 2020 Sep 15;259:112882. doi: 10.1016/j.jep.2020.112882. Epub 2020 Apr 20.
5
Effect of chitooligosaccharides with different degrees of acetylation on wheat seedlings under salt stress.不同乙酰化度壳寡糖对盐胁迫下小麦幼苗的影响。
Carbohydr Polym. 2015 Aug 1;126:62-9. doi: 10.1016/j.carbpol.2015.03.028. Epub 2015 Mar 21.
6
Chitosan Oligosaccharides Alleviate Colitis by Regulating Intestinal Microbiota and PPARγ/SIRT1-Mediated NF-κB Pathway.壳寡糖通过调节肠道微生物群和 PPARγ/SIRT1 介导的 NF-κB 通路缓解结肠炎。
Mar Drugs. 2022 Jan 24;20(2):96. doi: 10.3390/md20020096.
7
Attenuation of pro-inflammatory mediators in LPS-stimulated BV2 microglia by chitooligosaccharides via the MAPK signaling pathway.壳寡糖通过 MAPK 信号通路抑制 LPS 刺激的 BV2 小胶质细胞中促炎介质的产生。
Int J Biol Macromol. 2011 Nov 1;49(4):599-606. doi: 10.1016/j.ijbiomac.2011.06.014. Epub 2011 Jun 17.
8
The preparation, modification and hepatoprotective activity of chitooligosaccharides: A review.壳寡糖的制备、修饰及保肝活性：综述
Int J Biol Macromol. 2024 Oct;277(Pt 3):134489. doi: 10.1016/j.ijbiomac.2024.134489. Epub 2024 Aug 5.
9
Production and Characterization of Chitooligosaccharides: Evaluation of Acute Toxicity, Healing, and Anti-Inflammatory Actions.壳寡糖的生产与特性：急性毒性、愈合和抗炎作用评价。
Int J Mol Sci. 2021 Sep 30;22(19):10631. doi: 10.3390/ijms221910631.
10
Fyn kinase mediates pro-inflammatory response in a mouse model of endotoxemia: Relevance to translational research.Fyn 激酶在脂多糖血症小鼠模型中介导促炎反应：与转化研究的相关性。
Eur J Pharmacol. 2020 Aug 15;881:173259. doi: 10.1016/j.ejphar.2020.173259. Epub 2020 Jun 18.

引用本文的文献

1
Effect of Chitooligosaccharides on TLR2/NF-κB Signaling in LPS-Stimulated RAW 264.7 Macrophages.壳寡糖对脂多糖刺激的RAW 264.7巨噬细胞中TLR2/NF-κB信号传导的影响
Molecules. 2025 May 20;30(10):2226. doi: 10.3390/molecules30102226.
2
Comparison of the protective effects of chitosan oligosaccharides and chitin oligosaccharide on apoptosis, inflammation and oxidative stress.壳寡糖和甲壳素寡糖对细胞凋亡、炎症和氧化应激的保护作用比较。
Exp Ther Med. 2024 Jun 5;28(2):310. doi: 10.3892/etm.2024.12600. eCollection 2024 Aug.
3
Green-Chemical Strategies for Production of Tailor-Made Chitooligosaccharides with Enhanced Biological Activities.

本文引用的文献

1
Linagliptin Protects against Endotoxin-Induced Acute Kidney Injury in Rats by Decreasing Inflammatory Cytokines and Reactive Oxygen Species.利拉利汀通过降低炎症细胞因子和活性氧减轻内毒素诱导的大鼠急性肾损伤。
Int J Mol Sci. 2021 Oct 17;22(20):11190. doi: 10.3390/ijms222011190.
2
The Role of HSP70 in the Protective Effects of NVP-AUY922 on Multiple Organ Dysfunction Syndrome in Endotoxemic Rats.热休克蛋白70（HSP70）在内毒素血症大鼠中NVP-AUY922对多器官功能障碍综合征的保护作用中的角色
Front Pharmacol. 2021 Aug 6;12:724515. doi: 10.3389/fphar.2021.724515. eCollection 2021.
3
Mannose Receptor Mediates the Activation of Chitooligosaccharides on Blunt Snout Bream () Macrophages.
绿色化学策略用于生产具有增强生物活性的定制壳寡糖。
Molecules. 2023 Sep 13;28(18):6591. doi: 10.3390/molecules28186591.
4
Pleiotropic Modulation of Chitooligosaccharides on Inflammatory Signaling in LPS-Induced Macrophages.壳寡糖对脂多糖诱导的巨噬细胞炎症信号的多效性调节
Polymers (Basel). 2023 Mar 23;15(7):1613. doi: 10.3390/polym15071613.
甘露糖受体介导壳寡糖激活钝吻鲷（）巨噬细胞。
Front Immunol. 2021 Aug 2;12:686846. doi: 10.3389/fimmu.2021.686846. eCollection 2021.
4
Preparation and Antioxidant Activity of Chitosan Dimers with Different Sequences.不同序列壳聚糖二聚体的制备及抗氧化活性研究。
Mar Drugs. 2021 Jun 25;19(7):366. doi: 10.3390/md19070366.
5
Chitosan and chitooligosaccharides attenuate soyabean meal-induced intestinal inflammation of turbot (): possible involvement of NF-кB, activator protein-1 and mitogen-activated protein kinases pathways.壳聚糖和壳寡糖减轻大豆蛋白诱导的大菱鲆肠道炎症（）：可能涉及 NF-кB、激活蛋白-1 和丝裂原活化蛋白激酶途径。
Br J Nutr. 2021 Dec 14;126(11):1651-1662. doi: 10.1017/S0007114521000489. Epub 2021 Feb 8.
6
Anti-inflammatory activity of soluble chito-oligosaccharides (CHOS) on VitD3-induced human THP-1 monocytes.可溶性壳寡糖（CHOS）对 VitD3 诱导的人 THP-1 单核细胞的抗炎活性。
PLoS One. 2021 Feb 3;16(2):e0246381. doi: 10.1371/journal.pone.0246381. eCollection 2021.
7
Role of Metabolic Endotoxemia in Systemic Inflammation and Potential Interventions.代谢性内毒素血症在全身炎症中的作用及潜在干预措施
Front Immunol. 2021 Jan 11;11:594150. doi: 10.3389/fimmu.2020.594150. eCollection 2020.
8
Review: Advances in preparation of chitooligosaccharides with heterogeneous sequences and their bioactivity.综述：具有杂合序列的壳寡糖的制备及其生物活性的研究进展。
Carbohydr Polym. 2021 Jan 15;252:117206. doi: 10.1016/j.carbpol.2020.117206. Epub 2020 Oct 11.
9
Immunomodulatory Effects of -Acetyl Chitooligosaccharides on RAW264.7 Macrophages.-乙酰化壳寡糖对 RAW264.7 巨噬细胞的免疫调节作用。
Mar Drugs. 2020 Aug 12;18(8):421. doi: 10.3390/md18080421.
10
Chitoheptaose Promotes Heart Rehabilitation in a Rat Myocarditis Model by Improving Antioxidant, Anti-Inflammatory, and Antiapoptotic Properties.几丁七糖通过改善抗氧化、抗炎和抗细胞凋亡特性促进大鼠心肌炎模型的心脏康复。
Oxid Med Cell Longev. 2020 Apr 11;2020:2394704. doi: 10.1155/2020/2394704. eCollection 2020.