Huang Xin-Li, Zhou Jun-Lin, Zhou Xiao-Hong, Xian Xiao-Hui, Ding Chun-Hua
Department of Pathophysiology, Hebei Medical University, Shijiazhuang 050017, China.
Sheng Li Xue Bao. 2009 Oct 25;61(5):499-503.
To investigate the influence of sulfur dioxide (SO₂) on lipopolysaccharide (LPS)-induced acute lung injury (ALI), we examined the influence of exogenous SO₂ on pulmonary tissue inflammatory response. A rat model of ALI induced by intravenous (IV) injection of LPS was developed. Male Sprague-Dawley (SD) rats were divided into four groups randomly: control group, LPS group, LPS plus SO₂ group (IV injection of 0.5 mL Na₂SO₃/NaHSO₃ 10 min before LPS administration) and SO₂ group (only given Na₂SO₃/NaHSO₃). Animals were sacrificed 6 h after agent administration. Lung weight/body weight ratio (LW/BW) was measured and calculated. Morphological changes of lung tissues were observed. The number of polymorphonuclear neutrophil (PMN) in the bronchoalveolar lavage fluid (BALF), intercellular adhesion factor-1 (ICAM-1) expression in the lung tissue and IL-1, IL-6 and IL-10 levels in the serum were tested. The results showed that, compared to control rats, the LPS-treated rats had severe injuries of lung tissues and an increased LW/BW, increased index of quantitative assessment (IQA) score, increased PMN number in the BALF, increased ICAM-1 expression in the lung tissue and increased IL-1, IL-6 and IL-10 levels in the serum 6 h after LPS injection. Administration of the SO₂ donor, Na₂SO/₃NaHSO₃, into LPS-treated rats reduced the LW/BW, PMN number and ICAM-1 expression, and alleviated the degree of ALI (measured by the IQA score). In addition, Na₂SO₃/NaHSO₃ decreased IL-1 and IL-6 levels, but increased IL-10 level in the serum. There were no significant differences in the above indexes between SO₂-treated rats and control rats. These results suggest that exogenous SO₂ could inhibit the pulmonary tissue inflammatory response in rats with LPS-induced ALI.
为研究二氧化硫（SO₂）对脂多糖（LPS）诱导的急性肺损伤（ALI）的影响，我们检测了外源性SO₂对肺组织炎症反应的影响。建立了通过静脉注射LPS诱导ALI的大鼠模型。将雄性Sprague-Dawley（SD）大鼠随机分为四组：对照组、LPS组、LPS加SO₂组（在给予LPS前10分钟静脉注射0.5 mL Na₂SO₃/NaHSO₃）和SO₂组（仅给予Na₂SO₃/NaHSO₃）。给药6小时后处死动物。测量并计算肺湿重/体重比值（LW/BW）。观察肺组织的形态学变化。检测支气管肺泡灌洗液（BALF）中多形核中性粒细胞（PMN）数量、肺组织中细胞间黏附因子-1（ICAM-1）表达以及血清中IL-1、IL-6和IL-10水平。结果显示，与对照大鼠相比，LPS处理的大鼠肺组织有严重损伤，LW/BW增加，定量评估指数（IQA）评分增加，BALF中PMN数量增加，肺组织中ICAM-1表达增加，LPS注射6小时后血清中IL-1、IL-6和IL-10水平增加。向LPS处理的大鼠给予SO₂供体Na₂SO₃/NaHSO₃可降低LW/BW、PMN数量和ICAM-1表达，并减轻ALI程度（通过IQA评分衡量）。此外，Na₂SO₃/NaHSO₃降低了血清中IL-1和IL-6水平，但增加了IL-10水平。SO₂处理的大鼠与对照大鼠之间上述指标无显著差异。这些结果表明，外源性SO₂可抑制LPS诱导的ALI大鼠的肺组织炎症反应。
