Chen Yu-Qing, Rong Ling, Qiao Jian-Ou
Department of Respiratory Medicine, Shanghai First People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200080, P.R. China.
Department of Respiratory Medicine, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, P.R. China.
Mol Med Rep. 2014 Sep;10(3):1400-8. doi: 10.3892/mmr.2014.2328. Epub 2014 Jun 16.
This study investigated the effect of Panax notoginseng saponins (PNS) on acute lung injury (ALI) induced by oleic acid (OA) and lipopolysaccharide (LPS). A total of 28 Wistar rats were divided into four groups: sham; sham + PNS; OA‑LPS‑induced ALI and ALI + PNS. Lung tissue histology, lung wet‑to‑dry (W/D) weight ratio, extravascular lung water (EVLW) and epithelial sodium channel α (αENaC) mRNA and protein expression were examined. In addition, levels of inflammatory cytokines, including tumor necrosis factor α (TNF‑α), interleukin (IL)‑6 and IL‑10, as well as total leukocyte and neutrophil counts, were analyzed in rat bronchoalveolar lavage fluid (BALF) and serum. ALI + PNS rats were observed to exhibit significantly lower pulmonary parenchymal damage and EVLW compared with ALI rats. Furthermore, total leukocyte and neutrophil counts, and levels of inflammatory cytokines were significantly decreased following PNS administration in ALI rats. In addition, the decrease in αENaC mRNA and protein expression observed in the lung tissue of ALI rats was partially restored following PNS treatment. PNS treatment was demonstrated to ameliorate OA‑LPS‑induced ALI, potentially through restoration of αENaC mRNA and protein expression and through PNS‑induced anti‑inflammatory effects.
本研究调查了三七总皂苷（PNS）对油酸（OA）和脂多糖（LPS）诱导的急性肺损伤（ALI）的影响。将28只Wistar大鼠分为四组：假手术组；假手术 + PNS组；OA-LPS诱导的ALI组和ALI + PNS组。检测肺组织组织学、肺湿重与干重（W/D）比值、血管外肺水（EVLW）以及上皮钠通道α（αENaC）的mRNA和蛋白表达。此外，分析大鼠支气管肺泡灌洗液（BALF）和血清中炎症细胞因子的水平，包括肿瘤坏死因子α（TNF-α）、白细胞介素（IL）-6和IL-10，以及总白细胞和中性粒细胞计数。观察到ALI + PNS组大鼠与ALI组大鼠相比，肺实质损伤和EVLW显著降低。此外，ALI组大鼠给予PNS后，总白细胞和中性粒细胞计数以及炎症细胞因子水平显著降低。另外，ALI组大鼠肺组织中观察到的αENaC mRNA和蛋白表达的降低在PNS治疗后部分得到恢复。结果表明，PNS治疗可改善OA-LPS诱导的ALI，可能是通过恢复αENaC mRNA和蛋白表达以及PNS诱导的抗炎作用实现的。
