Department of Obstetrics and Gynecology, University Hospital Schleswig-Holstein, Campus Luebeck, Ratzeburger Allee 160, 23538, Luebeck, Germany.
Arch Gynecol Obstet. 2010 Jun;281(6):983-90. doi: 10.1007/s00404-009-1179-9. Epub 2009 Jul 15.
To analyze concentrations of endometrial leukocytes in patients with idiopathic-repeated abortions.
Biopsies of exactly dated secretory endometrium in 25 patients with idiopathic-repeated abortions and 10 control patients without a history of miscarriage were compared with respect to the concentrations of T-helper cells (CD4), cytotoxic T-cells (CD8), B-cells (CD19) and uterine natural killer cells (CD56) by immunohistochemistry and RNase protection assays.
All examined cells were detectable within secretory endometrium. No statistically significant differences of the examined immune-cell concentrations were seen between the control group and the repeated miscarriage group by either test.
This study suggests that the concentrations of specific endometrial leukocytes in a non-pregnant cycle are not associated with repeated pregnancy loss. Thus, the hypothesis of an altered endometrial immunity in patients with repeated miscarriages, symbolized by persistently differing local immune-cell concentrations, has to be questioned.
分析特发性复发性流产患者子宫内膜白细胞浓度。
对 25 例特发性复发性流产患者和 10 例无流产史的对照患者进行精确日期的分泌期子宫内膜活检，通过免疫组织化学和 RNase 保护试验比较 T 辅助细胞（CD4）、细胞毒性 T 细胞（CD8）、B 细胞（CD19）和子宫自然杀伤细胞（CD56）的浓度。
所有检测的细胞均在分泌期子宫内膜中可检测到。两种检测方法均未显示对照组和复发性流产组之间检查的免疫细胞浓度有统计学显著差异。
本研究表明，非妊娠周期特定子宫内膜白细胞的浓度与反复妊娠丢失无关。因此，反复流产患者子宫内膜免疫改变的假说，表现为持续不同的局部免疫细胞浓度，需要被质疑。
