Quenby S, Bates M, Doig T, Brewster J, Lewis-Jones D I, Johnson P M, Vince G
Division of Obstetrics and Gynaecology, City Hospital, Hucknall Road, Nottingham, UK.
Hum Reprod. 1999 Sep;14(9):2386-91. doi: 10.1093/humrep/14.9.2386.
Immunohistochemistry was used to investigate the leukocyte populations in the endometrium of women suffering recurrent miscarriage. Mid-luteal phase endometrial biopsies were taken from 22 patients with idiopathic recurrent miscarriage and from nine women with normal obstetric histories. The samples were dated histologically and stained with a panel of monoclonal antibodies to identify leukocytes. The outcome of any pregnancy in subsequent cycles following the biopsy was determined. Similar numbers of cluster designation (CD)3(+) and CD8(+) cells were seen in both groups. However, CD4(+), CD14(+), CD16(+), CD56(+) and MHC class II(+) cells were significantly higher in the recurrent miscarriage group than in the controls. Two patients had B cells (CD22(+)) in their endometrium. No CD57(+) cells were seen in the controls; however, eight of the patients had a few CD57(+) cells present. Only two patients, both from the recurrent miscarriage group, had CD69(+) leukocytes in their endometrium. Patients who had miscarriages following endometrial biopsy had significantly more CD4(+), CD8(+), CD14(+), CD16(+), and CD56(+) leukocytes in their endometrium than either those who had live births or women with proven fertility. A different population of leukocytes was found in the pre-implantation endometrium from recurrent miscarriage patients as compared to those from fertile controls. These differences were accentuated in women who had a miscarriage subsequent to the biopsy compared with those who subsequently had a live birth.
采用免疫组织化学方法研究复发性流产女性子宫内膜中的白细胞群体。从22例特发性复发性流产患者及9例有正常产科病史的女性中获取黄体中期子宫内膜活检组织。对样本进行组织学分期，并用一组单克隆抗体染色以识别白细胞。确定活检后随后周期中任何妊娠的结局。两组中可见相似数量的簇分化抗原（CD）3(+)和CD8(+)细胞。然而，复发性流产组中CD4(+)、CD14(+)、CD16(+)、CD56(+)和MHC II类(+)细胞显著高于对照组。两名患者的子宫内膜中有B细胞（CD22(+)）。对照组中未见CD57(+)细胞；然而，8例患者有少量CD57(+)细胞。仅两名患者（均来自复发性流产组）的子宫内膜中有CD69(+)白细胞。子宫内膜活检后发生流产的患者，其子宫内膜中的CD4(+)、CD8(+)、CD14(+)、CD16(+)和CD56(+)白细胞显著多于分娩活婴者或已证实有生育能力的女性。与有生育能力的对照组相比，复发性流产患者的植入前子宫内膜中发现了不同的白细胞群体。与随后分娩活婴的女性相比，活检后发生流产的女性中这些差异更为明显。
