Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, TX 76019-0065, USA.
Electrophoresis. 2009 Nov;30(22):3870-6. doi: 10.1002/elps.200900299.
Detection of microbial contamination is of critical importance in the medical and the food industry. Rapid tests for the absence or presence of viable microorganisms are in urgent demand. CE is a modern analytical technique that can be adapted for rapid screening of microbial contamination. However, the small dimensions of capillaries allow the introduction of only a small fraction of the sample, which can be problematic when examining large samples. In this article, we examine the possibilities of introducing larger sample volumes using capillaries with greater id together with different stacking techniques. The use of 0.32 mm id capillary and the injection of 60% of the capillary volume led to approximately 120-fold improvement of the injected sample volume over the classical injection 5% of a 0.10-mm id capillary. The setup we described opens new possibilities in sterility testing using CE.
微生物污染的检测在医学和食品工业中至关重要。人们迫切需要能够快速检测是否存在有活力的微生物的方法。CE 是一种现代分析技术，可以用于快速筛选微生物污染。然而，由于毛细管的尺寸较小，只能引入样品的一小部分，这在检测大样本时可能会成为问题。在本文中，我们研究了使用内径较大的毛细管和不同的堆积技术引入更大体积样品的可能性。使用 0.32 毫米内径的毛细管，并注入 60%的毛细管体积，与经典的 0.10 毫米内径毛细管注入 5%相比，注入样品的体积提高了约 120 倍。我们所描述的设置为使用 CE 进行无菌检测开辟了新的可能性。
