Bao Ye, Lantz Andrew W, Crank Jeffrey A, Huang Junmin, Armstrong Daniel W
Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, TX, USA.
Electrophoresis. 2008 Jun;29(12):2587-92. doi: 10.1002/elps.200700719.
A rapid test of whether a laboratory sample contains any microorganisms is important and necessary for many areas of science and technology. Currently, most of the standard procedures for the detection of aerobic bacteria, anaerobic bacteria and fungi, require the preparation of microbial cultures in respective growth media, which are dramatically slow. Different approaches providing fast analysis such as CE are becoming more desired. To compensate for the natural electrophoretic heterogeneity of microbes, various buffer additives were examined to stack all bacteria and fungi in a sample plug into a single peak. This peak was removed from the molecular contaminants in the sample to prevent false positives. Both cationic surfactants and ionic liquids (IL) were investigated as run buffer additives and they are both widely applicable to different species of bacteria and fungi. Given that high concentrations of surfactants can potentially lyse cells, dicationic IL offer attractive auxiliary buffer additives for use in CE-based sterility tests. The analysis can be completed in 10 min, thus providing a great advantage over traditional direct inoculation methods that require several weeks to complete.
对于许多科学技术领域而言，快速检测实验室样品中是否含有任何微生物既重要又必要。目前，大多数检测需氧菌、厌氧菌和真菌的标准程序都要求在各自的生长培养基中制备微生物培养物，这一过程极其缓慢。像毛细管电泳（CE）这样能够提供快速分析的不同方法越来越受到青睐。为了弥补微生物天然的电泳异质性，人们研究了各种缓冲添加剂，以便将样品塞中的所有细菌和真菌堆积成一个单一峰。该峰与样品中的分子污染物分离，以防止出现假阳性。阳离子表面活性剂和离子液体（IL）都作为运行缓冲添加剂进行了研究，它们都广泛适用于不同种类的细菌和真菌。鉴于高浓度的表面活性剂可能会使细胞裂解，双阳离子离子液体为基于毛细管电泳的无菌测试提供了有吸引力的辅助缓冲添加剂。该分析可在10分钟内完成，因此与需要数周才能完成的传统直接接种方法相比具有很大优势。
