Department of Gastrointestinal Sciences, Christian Medical College, Vellore 632004, India.
Br J Nutr. 2010 Feb;103(3):335-8. doi: 10.1017/S0007114509992182. Epub 2009 Oct 23.
Gut bacteria contribute to energy conservation in man through their ability to ferment unabsorbed carbohydrate. The present study examined the composition of predominant faecal microbiota in obese and non-obese children. The participants (n 28) aged 11-14 years provided fresh faecal samples and completed a dietary survey consisting of 24 h diet recall and a FFQ of commonly used foods taken over the previous 3 months. Faecal bacteria were quantitated by real-time PCR using primers targeted at 16S rDNA. Of the participants, fifteen (seven female) were obese, with median BMI-for-age at the 99th percentile (range 97 to>99) while thirteen participants (seven female) were normal weight, with median BMI-for age being at the 50th percentile (range 1-85). Consumption of energy, carbohydrates, fat and protein was not significantly different between the obese and non-obese participants. There was no significant difference between the two groups in faecal levels of Bacteroides-Prevotella, Bifidobacterium species, Lactobacillus acidophilus group or Eubacterium rectale. Levels of Faecalibacterium prausnitzii were significantly higher in obese children than in non-obese participants (P = 0.0253). We concluded that the finding of increased numbers of F. prausnitzii in the faeces of obese children in south India adds to the growing information on alterations in faecal microbiota in obesity.
肠道细菌通过发酵未被吸收的碳水化合物的能力为人体提供能量。本研究检测了肥胖和非肥胖儿童主要粪便微生物群的组成。参与者（n=28）年龄在 11-14 岁之间，提供新鲜粪便样本，并完成了包括 24 小时饮食回顾和过去 3 个月常用食物的 FFQ 的饮食调查。使用针对 16S rDNA 的引物通过实时 PCR 定量粪便细菌。在参与者中，有十五名（七名女性）肥胖，体重指数年龄中位数位于第 99 百分位数（范围为 97 至>99），而十三名参与者（七名女性）体重正常，体重指数年龄中位数位于第 50 百分位数（范围为 1-85）。肥胖和非肥胖参与者的能量、碳水化合物、脂肪和蛋白质的摄入量没有显著差异。两组粪便中双歧杆菌属、双歧杆菌属、嗜酸乳杆菌群或直肠真杆菌的水平没有显著差异。肥胖儿童粪便中普氏粪杆菌的水平明显高于非肥胖参与者（P=0.0253）。我们的结论是，在印度南部肥胖儿童粪便中发现普氏粪杆菌数量增加，这增加了肥胖症中粪便微生物群改变的信息。
