School of Dental Hygiene, China Medical University, Taichung, Taiwan, Republic of China.
Hum Exp Toxicol. 2009 Dec;28(12):785-90. doi: 10.1177/0960327109350670.
It is well documented that enhanced garlic (Allium sativum) consumption leads to decrease in the cancer incidences. Diallyl sulfide (DAS), one of the components of garlic, induces cytotoxicity and apoptosis in many cancer cell lines. The present studies are focused on the in vivo effects of DAS on leukemia WEHI-3 cells in the BALB/c mice. We examined the effects of DAS on the cytotoxicity of WEHI-3 cells and results indicated that DAS decreased the percentage of viable WEHI-3 cells and these effects are dose-dependent. We examined the effects of DAS on WEHI-3 in vivo and the results indicated that DAS decreased the percentage of Mac-3 and CD11b, indicating that the differentiation of the precursor of macrophage cells was inhibited. DAS stimulated the percentage of CD3 and CD19, indicating that the differentiation of the precursor of T and B cells promoted. The weights of liver and spleen indicated that DAS decreased the weight of these organs after being compared to the control groups. One of the major characteristic of WEHI-3 leukemia is the enlarged spleen in murine after intraperitoneal (i.p.) injection of WEHI-3 cells. In conclusion, DAS affects WEHI-3 cells both in vitro and in vivo.
有大量文献记载，增加大蒜（Allium sativum）的摄入量可降低癌症发病率。大蒜的成分之一二烯丙基二硫（Diallyl sulfide，DAS）可诱导许多癌细胞系产生细胞毒性和细胞凋亡。本研究主要关注 DAS 对 BALB/c 小鼠 WEHI-3 白血病细胞的体内作用。我们研究了 DAS 对 WEHI-3 细胞的细胞毒性作用，结果表明 DAS 降低了活细胞的百分比，且呈剂量依赖性。我们研究了 DAS 对 WEHI-3 细胞在体内的作用，结果表明 DAS 降低了 Mac-3 和 CD11b 的百分比，表明巨噬细胞前体细胞的分化受到抑制。DAS 刺激 CD3 和 CD19 的百分比，表明 T 和 B 细胞前体细胞的分化得到了促进。肝脏和脾脏的重量表明，与对照组相比，DAS 降低了这些器官的重量。WEHI-3 白血病的一个主要特征是 WEHI-3 细胞腹腔注射后小鼠脾脏肿大。总之，DAS 对 WEHI-3 细胞具有体内外作用。
