Yang Jai-Sing, Kok Lai-Fong, Lin Yu-Hsuan, Kuo Tzu-Ching, Yang Jiun-Long, Lin Chin-Chung, Chen Guang-Wei, Huang Wen-Wen, Ho Heng-Chien, Chung Jing-Gung
Department of Medical Technology Yuan-Pei University of Science and Technology, Hsinchu, Taiwan, ROC.
Anticancer Res. 2006 Jan-Feb;26(1A):219-25.
Enhanced garlic (Allium sativum) consumption is closely related to reduced cancer incidence, as shown in epidemiological studies. Diallyl disulfide (DADS), a component of garlic, inhibits the proliferation of human blood, colon, lung and skin cancer cells. Although DADS had been reported to induce apoptosis in human leukemia HL-60 cells, there are no reports regarding whether or not it affects leukemia cells in vivo. Therefore, the present study is focused on the in vivo effects of DADS on WEHI-3 leukemia cells. The effects of DADS on murine WEHI-3 cells were initially examined, and the results indicated that DADS induced cytotoxicity and that this effect was dose-dependent. The effects of DADS on WEHI-3 in BALBIc mice were also examined, and the results indicated that DADS decreased the percentage of MAC-3 marker, indicating that differentiation of the precursor of macrophage and T cells was inhibited. The weights of liver and spleen were also measured, and the results indicated that DADS decreased the weight of these organs. An important characteristic of WEHI-3 leukemia is the enlarged spleen in mice after i.p. injection of WEHI-3 cells. Based on pathological examination, the function of DADS was observed in the liver and spleen of mice previously injected with WEHI-3 cells. Apparently, DADS affects WEHI-3 cells both in vitro and in vivo.
流行病学研究表明，增加大蒜（葱属植物）的摄入量与降低癌症发病率密切相关。大蒜的一种成分二烯丙基二硫醚（DADS）可抑制人类血液、结肠、肺和皮肤癌细胞的增殖。尽管已有报道称DADS可诱导人白血病HL - 60细胞凋亡，但尚无关于其在体内是否影响白血病细胞的报道。因此，本研究聚焦于DADS对WEHI - 3白血病细胞的体内作用。首先检测了DADS对小鼠WEHI - 3细胞的作用，结果表明DADS可诱导细胞毒性，且这种作用呈剂量依赖性。还检测了DADS对BALBIc小鼠体内WEHI - 3细胞的作用，结果表明DADS降低了MAC - 3标志物的百分比，表明巨噬细胞和T细胞前体的分化受到抑制。同时测量了肝脏和脾脏的重量，结果表明DADS使这些器官的重量减轻。WEHI - 3白血病的一个重要特征是腹腔注射WEHI - 3细胞后小鼠脾脏肿大。基于病理检查，观察到DADS对先前注射过WEHI - 3细胞的小鼠肝脏和脾脏有作用。显然，DADS在体外和体内均对WEHI - 3细胞有影响。
