Kim Do Young, Park Doo-Sang, Kwon Soon Bum, Chung Moon Gyu, Bae Kyung Sook, Park Ho-Yong, Rhee Young Ha
Industrial Bio-materials Research Center, KRIBB, Daejeon, Republic of Korea.
J Microbiol. 2009 Oct;47(5):651-6. doi: 10.1007/s12275-009-0109-7. Epub 2009 Oct 24.
Burkholderia sp. IS-01 capable of biosynthesizing poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [poly(3HB-co-3HV)] copolyesters with a high molar fraction of 3HV was isolated from the gut of the adult longicorn beetle, Moechotypa diphysis. The strain IS-01 was relatively tolerant to high concentrations of levulinic acid and accumulated a poly(13.5 mol% 3HB-co-86.5 mol% 3HV) copolyester when cultivated on a mixture of gluconate (20 g/L) and levulinic acid (12.5 g/L). In this case, the content of the copolyester in the cells was approximately 60.0%. The compositions of the copolyesters were easily regulated by altering the molar ratio of gluconate and levulinic acid in the medium. The organism was found to possess a class I PHA synthase (PhaC) gene (1,881 bp) that encodes a protein with a deduced molecular mass of 68,538 Da that consists of 626 amino acids. The PhaC of this organism was most similar to that of B. cenocepacia PC184 (92% similarity).
从成年天牛双斑锦天牛的肠道中分离出了能够生物合成具有高3HV摩尔分数的聚（3-羟基丁酸酯-co-3-羟基戊酸酯）[聚（3HB-co-3HV）]共聚酯的伯克霍尔德氏菌属IS-01。菌株IS-01对高浓度的乙酰丙酸具有相对耐受性，当在葡萄糖酸盐（20 g/L）和乙酰丙酸（12.5 g/L）的混合物上培养时，积累了聚（13.5 mol% 3HB-co-86.5 mol% 3HV）共聚酯。在这种情况下，细胞中共聚酯的含量约为60.0%。通过改变培养基中葡萄糖酸盐和乙酰丙酸的摩尔比，可以很容易地调节共聚酯的组成。发现该生物体拥有一个I类PHA合酶（PhaC）基因（1,881 bp），该基因编码一种推导分子量为68,538 Da、由626个氨基酸组成的蛋白质。该生物体的PhaC与洋葱伯克霍尔德菌PC184的PhaC最相似（相似度为92%）。
