Key laboratory of Protein Chemistry and Developmental Biology, Ministry of Education of China, Department of Biochemistry and Molecular Biology, College of Life Science, Hunan Normal University, Changsha, China.
Mol Biol Rep. 2010 Jul;37(6):2967-73. doi: 10.1007/s11033-009-9863-1. Epub 2009 Oct 23.
TNFAIP1 is a protein which can be induced by tumor necrosis factoralpha (TNFalpha) and interleukin-6 (IL-6), it may play roles in DNA synthesis, DNA repair, cell apoptosis and human diseases. However, very little has been known about how TNFAIP1 acts in these physiological processes. In this paper, CK2beta was identified as a partner of TNFAIP1 by screening the HeLa cDNA library in yeast two-hybrid system with TNFAIP1 as a bait. Furthermore, it was demonstrated that CK2 could phosphorylate TNFAIP1 in vitro and in vivo, which facilitated the distribution of TNFAIP1 in nucleus and enhanced its interaction with PCNA. It is suggested that the phosphorylation of TNFAIP1 may be required for its functions.
TNFAIP1 是一种可被肿瘤坏死因子α（TNFα）和白细胞介素-6（IL-6）诱导的蛋白，它可能在 DNA 合成、DNA 修复、细胞凋亡和人类疾病中发挥作用。然而，关于 TNFAIP1 在这些生理过程中如何发挥作用的信息知之甚少。在本文中，我们通过酵母双杂交系统用 TNFAIP1 作为诱饵筛选 HeLa cDNA 文库，鉴定出 CK2β是 TNFAIP1 的一个伴侣。此外，我们还证明 CK2 可以在体外和体内使 TNFAIP1 磷酸化，这促进了 TNFAIP1 在核内的分布，并增强了它与 PCNA 的相互作用。这表明 TNFAIP1 的磷酸化可能是其发挥功能所必需的。
