Institute for Environmental and Gender-specific Medicine, Juntendo University Graduate School of Medicine, Urayasu, Chiba 279-0023, Japan.
FEBS Lett. 2010 May 3;584(9):1642-52. doi: 10.1016/j.febslet.2009.10.043. Epub 2009 Oct 21.
Lactosylceramide (LacCer), a neutral glycosphingolipid, is abundantly expressed on human neutrophils, and specifically recognizes several pathogenic microorganisms. LacCer forms membrane microdomains coupled with the Src family kinase Lyn on the plasma membrane, and ligand binding to LacCer activates Lyn, resulting in neutrophil functions. In contrast, neutrophilic differentiated HL-60 cells do not have Lyn-associated LacCer-enriched microdomains and lack LacCer-mediated functions. In neutrophil plasma membranes, the very long fatty acid C24:0 and C24:1 chains are the main components of LacCer, whereas plasma membrane of D-HL-60 cells mainly includes C16-LacCer species. Here, we suggest that LacCer species containing very long fatty acid chains are indispensable for the association of Lyn with LacCer-enriched microdomains and LacCer-mediated functions.
乳糖基神经酰胺 (LacCer) 是一种中性糖脂，在人中性粒细胞中大量表达，并且特异性识别几种致病微生物。LacCer 与质膜上的Src 家族激酶 Lyn 一起形成膜微区，配体与 LacCer 结合可激活 Lyn，从而导致中性粒细胞的功能。相比之下，分化的 HL-60 中性粒细胞没有与 Lyn 相关的富含 LacCer 的微区，并且缺乏 LacCer 介导的功能。在中性粒细胞质膜中，非常长的脂肪酸 C24:0 和 C24:1 链是 LacCer 的主要成分，而 D-HL-60 细胞的质膜主要包含 C16-LacCer 种类。在这里，我们提出含有非常长脂肪酸链的 LacCer 种类对于 Lyn 与富含 LacCer 的微区的关联以及 LacCer 介导的功能是必不可少的。
