Sonnino Sandro, Prinetti Alessandro, Nakayama Hitoshi, Yangida Mitsuaki, Ogawa Hideoki, Iwabuchi Kazuhisa
Dipartimento di Chimica, Biochimica e Biotecnologie per la Medicina, Università degli Studi di Milano, Via Fratelli Cervi 93, Segrate, Italy.
Glycoconj J. 2009 Aug;26(6):615-21. doi: 10.1007/s10719-008-9215-8. Epub 2008 Nov 18.
Glycosphingolipids are highly enriched in specialized membrane microdomains ("lipid rafts", caveolar domains and glycosynapses), and they participate to the process of transduction of information across the membrane. Lactosylceramide (LacCer) is specifically coupled with the Src family kinase Lyn in plasma membrane microdomains of human neutrophils. Ligand binding to LacCer activates Lyn, resulting in neutrophil functions, such as superoxide generation and migration. The beta-Gal-(1-4)-beta-Glc disaccharide structure of LacCer is necessary, but it is not sufficient for LacCer-mediated Lyn activation. For this function, the presence of a LacCer molecular species with ceramide containing a very long fatty acid chain is also required. In this manuscript, we discuss the importance of interdigitation within the membrane, promoted by the presence of glycosphingolipid species with very long fatty acyl chains as determinants for membrane organization, instrumental to the signaling process.
糖鞘脂在特殊的膜微区（“脂筏”、小窝结构域和糖突触）中高度富集，并且它们参与跨膜信息转导过程。乳糖神经酰胺（LacCer）在人中性粒细胞的质膜微区中与Src家族激酶Lyn特异性结合。配体与LacCer结合会激活Lyn，从而导致中性粒细胞发挥功能，如超氧化物生成和迁移。LacCer的β - Gal-(1-4)-β - Glc二糖结构是必需的，但对于LacCer介导的Lyn激活来说并不充分。对于此功能，还需要存在含有极长脂肪酸链的神经酰胺的LacCer分子种类。在本手稿中，我们讨论了由含有极长脂肪酰链的糖鞘脂种类的存在所促进的膜内相互交错作为膜组织决定因素的重要性，这对信号转导过程至关重要。
