Instituto de Investigaciones Biológicas-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, cc1245, 7600 Mar del Plata, Argentina.
Fungal Genet Biol. 2010 Jan;47(1):53-65. doi: 10.1016/j.fgb.2009.10.008.
The ascomycete plant pathogen Botrytis cinerea secretes aspartic proteinase (AP) activity. Functional analysis was carried out on five aspartic proteinase genes (Bcap1-5) reported previously. Single and double mutants lacking these five genes showed neither a reduced secreted proteolytic activity, nor a reduction in virulence and they showed no alteration in sensitivity to antifungal proteins purified from grape juice. Scrutiny of the B. cinerea genome revealed the presence of nine additional Bcap genes, denoted Bcap6-14. The product of the Bcap8 gene was found to constitute up to 23% of the total protein secreted by B. cinerea. Bcap8-deficient mutants secreted approximately 70% less AP activity but were just as virulent as the wild-type strain. Phylogenetic analysis showed that Bcap8 has orthologs in many basidiomycetes but only few ascomycetes including the biocontrol fungus Trichoderma harzanium. Potential functions of the 14 APs in B. cinerea are discussed based on their sequence characteristics, phylogeny and predicted localization.
子囊菌植物病原菌 Botrytis cinerea 分泌天冬氨酸蛋白酶 (AP) 活性。对以前报道的五个天冬氨酸蛋白酶基因 (Bcap1-5) 进行了功能分析。缺乏这五个基因的单突变体和双突变体既没有降低分泌的蛋白水解活性，也没有降低毒力，它们对从葡萄汁中纯化的抗真菌蛋白也没有改变敏感性。对 B. cinerea 基因组的仔细研究揭示了存在另外九个 Bcap 基因，分别表示为 Bcap6-14。Bcap8 基因的产物构成了 B. cinerea 分泌的总蛋白的 23%。Bcap8 缺陷突变体分泌的 AP 活性约减少 70%，但与野生型菌株一样具有毒力。系统发育分析表明，Bcap8 在许多担子菌中具有同源物，但只有少数子囊菌包括生防真菌哈茨木霉具有同源物。基于它们的序列特征、系统发育和预测的定位，讨论了 B. cinerea 中 14 种 AP 的潜在功能。
