分散的胰腺腺泡细胞淀粉酶释放的调节。
Regulation of amylase release from dispersed pancreatic acinar cells.
作者信息
Gardner J D, Jackson M J
出版信息
J Physiol. 1977 Sep;270(2):439-54. doi: 10.1113/jphysiol.1977.sp011961.
Abstract
- A study has been made of factors influencing release of amylase from dispersed pancreatic acinar cells. 2. In the basal, unstimulated, condition cells released 2-3% of the total amylase present in 30 min. 3. The rate of amylase release was stimulated 50-70% by C-terminal octapeptide of cholecystokinin (CCK-OP, maximally effective concentration, 3 X 10(-10) M); carbamylcholine (maximally effective concentration, 10(-5 M); secretin (maximally effective concentration greater than 10(-6) M); vasoactive intestinal peptide (VIP, maximally effective concentration, 10(-8) M); and adenosine 3':5' monophosphate (cyclic AMP) and guanosine 3':5' monophosphate (cyclic GMP) as well as their dibutyryl derivatives (maximally effective concentrations, 10(-3) M). 4. The responses to CCK-OP or carbamylcholine were potentiated by secretin, VIP or dibutyryl cyclic AMP. 5. The responses to secretin or VIP were potentiated by CCK-OP, carbamylcholine, or dibutyryl cyclic GMP. 6. There appear to be two pathways for the regulation of amylase release from pancreatic acinar cells: one pathway can be stimulated by cholecystokinin or cholinergic agonists, and the response to these stimuli is mediated by cyclic GMP; the other pathway can be stimulated by secretin or VIP, and the response to these stimuli is mediated by cyclic AMP.
摘要
- 对影响分散的胰腺腺泡细胞淀粉酶释放的因素进行了研究。2. 在基础的、未受刺激的状态下，细胞在30分钟内释放了其所含总淀粉酶的2 - 3%。3. 胆囊收缩素的C末端八肽（CCK - OP，最大有效浓度为3×10⁻¹⁰ M）、氨甲酰胆碱（最大有效浓度为10⁻⁵ M）、促胰液素（最大有效浓度大于10⁻⁶ M）、血管活性肠肽（VIP，最大有效浓度为10⁻⁸ M）以及腺苷3':5' - 单磷酸（环磷酸腺苷）和鸟苷3':5' - 单磷酸（环磷酸鸟苷）及其二丁酰衍生物（最大有效浓度为10⁻³ M）可使淀粉酶释放速率提高50 - 70%。4. 促胰液素、VIP或二丁酰环磷酸腺苷可增强对CCK - OP或氨甲酰胆碱的反应。5. CCK - OP、氨甲酰胆碱或二丁酰环磷酸鸟苷可增强对促胰液素或VIP的反应。6. 胰腺腺泡细胞淀粉酶释放的调节似乎存在两条途径：一条途径可被胆囊收缩素或胆碱能激动剂刺激，对这些刺激的反应由环磷酸鸟苷介导；另一条途径可被促胰液素或VIP刺激，对这些刺激的反应由环磷酸腺苷介导。
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