Connecticut Comprehensive Center for Hernia Repair, Department of Surgery, University of Connecticut Health Center, Farmington, Connecticut 06030, USA.
J Surg Res. 2010 Jan;158(1):10-4. doi: 10.1016/j.jss.2009.05.033.
Inflammation and wound healing play critical roles in the integration of biologic meshes (BMs) at sites of hernia repair. Monocytes/macrophages (M/MQs) are key cells involved in mesh integration. Interleukin-1beta (IL-1beta) is one of the major M/MQ-derived cytokines, and its expression is a reflection of the degree of M/MQ activation. We hypothesized that BMs induce M/MQ activation in vitro and that IL-1beta expression by M/MQ varies among various BMs.
Acellular human dermis-derived BM samples (AlloDerm, AlloMax, FlexHD) were placed in 48-well plates and cultured with peripheral blood mononuclear cells (PBMCs) from three healthy human subjects for 7 d. The resulting supernatants were assayed for IL-1beta levels by enzyme-linked immunosorbent assay (ELISA), and the BMs were evaluated histologically.
IL-1beta expression varied among donors as well as the BMs [AlloDerm (2.11-38.25pg/10(6) PBMCs); AlloMax (13.12-715.40pg/10(6) PBMCs); and FlexHD (116.69-665.40pg/10(6) PBMCs)]. Analysis of this data indicated that AlloMax and FlexHD induced significantly more M/MQ activation compared with AlloDerm (P<0.05). Histologic evaluation of the BMs indicated adherence of M/MQs on BM surface, however no degradation was detected.
For the first time, we have demonstrated that M/MQs are activated to varying levels by human BMs in vitro. These differences may be related to BM processing technologies and/or the biologic variation between donors. Our results raise the possibility that these differences in M/MQ activation could result in varying intensity of inflammation and wound healing that control the integration of BMs at sites of hernia repair.
炎症和伤口愈合在疝修补部位生物补片（BM）的整合中起着关键作用。单核细胞/巨噬细胞（M/MQ）是参与补片整合的关键细胞。白细胞介素-1β（IL-1β）是 M/MQ 衍生细胞因子的主要成分之一，其表达反映了 M/MQ 激活的程度。我们假设 BM 在体外诱导 M/MQ 激活，并且 M/MQ 对不同 BM 的 IL-1β 表达存在差异。
将脱细胞人真皮衍生 BM 样本（AlloDerm、AlloMax、FlexHD）置于 48 孔板中，并与来自 3 名健康供体的外周血单核细胞（PBMC）共培养 7d。通过酶联免疫吸附试验（ELISA）检测上清液中 IL-1β 水平，并对 BM 进行组织学评估。
IL-1β 表达在供体之间以及 BM 之间存在差异[AlloDerm（2.11-38.25pg/106 PBMC）；AlloMax（13.12-715.40pg/106 PBMC）；FlexHD（116.69-665.40pg/106 PBMC）]。对该数据的分析表明，与 AlloDerm 相比，AlloMax 和 FlexHD 诱导的 M/MQ 激活显著更多（P<0.05）。对 BM 的组织学评估表明 M/MQ 黏附在 BM 表面，但未检测到降解。
我们首次证明 M/MQ 在体外被人 BM 以不同水平激活。这些差异可能与 BM 处理技术和/或供体之间的生物学差异有关。我们的结果提出了这样一种可能性，即这些 M/MQ 激活的差异可能导致炎症和伤口愈合的强度不同，从而控制疝修补部位 BM 的整合。
