Institute of Spectroscopy, Russian Academy of Sciences, Troitsk, Moscow Region, Russia.
Biosens Bioelectron. 2010 Jan 15;25(5):1212-6. doi: 10.1016/j.bios.2009.09.011. Epub 2009 Sep 18.
A high-precision optical biosensor technique capable of independently determining the refractive index (RI) of liquids is presented. Photonic crystal surface waves were used to detect surface binding events, while an independent registration of the critical angle was used for accurate determination of the liquid RI. This technique was tested using binding of biotin molecules to a streptavidin monolayer at low and high biotin concentrations. The attained baseline noise is 5x10(-13) m/Hz(1/2) for adlayer thickness changes and 9x10(-8) RIU/Hz(1/2) for RI changes.
提出了一种能够独立测定液体折射率（RI）的高精度光学生物传感器技术。该技术利用光子晶体表面波来检测表面结合事件，同时独立记录临界角，以准确测定液体 RI。该技术在低浓度和高浓度生物素分子与链霉亲和素单层结合的情况下进行了测试。对于覆盖层厚度变化，获得的基线噪声为 5x10(-13) m/Hz(1/2)，对于 RI 变化，获得的基线噪声为 9x10(-8) RIU/Hz(1/2)。
