Pharmacy Department, School of Pharmaceutical Sciences, University of Sao Paulo, Av. Prof. Lineu Prestes 580, 05508-000 Sao Paulo, SP, Brazil.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Dec 1;877(31):3960-4. doi: 10.1016/j.jchromb.2009.10.005. Epub 2009 Oct 9.
A sensitive and rapid HPLC assay for determining cefuroxime penetration in the subcutaneous tissue near to surgical incision of patients submitted to coronary artery bypass grafting (CABG) with or without cardiopulmonary bypass (CPB) was performed. Blood and subcutaneous tissue samples were collected from 14 patients, in four periods during surgery. The analytical method presented linearity from 0.5 to 100 microg/g, LOQ=0.50 microg/g, LOD=0.25 microg/g, intra- and interday precision (%CV) ranged from 4.9 to 8.9% and 6.4 to 9.9%, respectively, and intra- and interday accuracy expressed as % of the nominal concentration ranged from 87.1 to 104.6% and 94.8 to 103.8%, respectively (mean of three concentrations). Relative recovery was 98.4%. Tissue/plasma ratios obtained for CPB and non-CPB were, respectively: 14.6% vs 19.0% (0.6 h); 15.7% vs 15.7% (2.1 h); 22.5% vs 19.9% (3.6 h); 15.7% vs 18.8% (4.5 h). Data obtained indicate that tissue/plasma ratio remains unchanged in CPB and non-CPB patients during all period of surgery and the CPB does not affect the penetration of cefuroxime in tissues close to the surgical wound.
一种灵敏、快速的 HPLC 分析方法，用于测定行冠状动脉旁路移植术（CABG）患者手术切口附近皮下组织中头孢呋辛的浓度，这些患者行或不行心肺转流术（CPB）。对 14 名患者的血液和皮下组织样本进行了收集，分别在手术的四个时期。该分析方法的线性范围为 0.5-100μg/g，定量下限（LOQ）=0.50μg/g，定量下限（LOD）=0.25μg/g，日内和日间精密度（%CV）分别为 4.9-8.9%和 6.4-9.9%，日内和日间准确度（以名义浓度的%表示）分别为 87.1-104.6%和 94.8-103.8%（三个浓度的平均值）。相对回收率为 98.4%。CPB 和非-CPB 患者的组织/血浆比值分别为：14.6%比 19.0%（0.6 小时）；15.7%比 15.7%（2.1 小时）；22.5%比 19.9%（3.6 小时）；15.7%比 18.8%（4.5 小时）。所得数据表明，在整个手术期间，CPB 和非-CPB 患者的组织/血浆比值保持不变，CPB 并不影响头孢呋辛在手术切口附近组织中的渗透。
