Departamento de Química, Universidade Federal do Rio Grande do Norte, Campus Universitário, Lagoa Nova, Natal, RN 59078-970, Brazil.
Carbohydr Res. 2009 Dec 14;344(18):2591-5. doi: 10.1016/j.carres.2009.08.030. Epub 2009 Aug 28.
Chitosan is a polysaccharide used in a broad range of applications. Many of its unique properties come from the presence of amino groups in its structure. A proper quantification of these amino groups is very important, in order to specify if a given chitosan sample can be used in a particular application. In this work, a comparison between the determination of chitosan degree of deacetylation by conductometry and CHN elemental analysis was carried out, using a rigorous error analysis. Accurate expressions relating CHN composition, conductometric titration, and degree of deacetylation, in conjunction with their associated errors, were developed and reported in this note. Error analysis showed conductometric analysis as an inexpensive and secure method for the determination of the degree of deacetylation of chitosan.
壳聚糖是一种在广泛应用中使用的多糖。其许多独特的性质来自于其结构中存在的氨基。为了指定特定应用中是否可以使用给定的壳聚糖样品，对这些氨基进行适当的定量非常重要。在这项工作中，通过严格的误差分析，对电导率法和 CHN 元素分析测定壳聚糖脱乙酰度进行了比较。本文还开发并报告了有关 CHN 组成、电导率滴定和脱乙酰度的准确表达式及其相关误差。误差分析表明，电导率分析是一种用于测定壳聚糖脱乙酰度的廉价且安全的方法。
