Research Institute for Bioresources, Okayama University, Kurashiki, Okayama 710-0046, Japan.
J Plant Physiol. 2010 Mar 15;167(5):358-64. doi: 10.1016/j.jplph.2009.09.011. Epub 2009 Oct 23.
The growth kinetics in the presence of copper (Cu) of the protonema of the moss Scopelophila cataractae and the matrix polysaccharides of its cell walls have been analyzed in this study. Protonemal cells cultured in a medium containing 0.2mM CuSO(4) showed a rapid accumulation of Cu, reaching a maximum between 30 and 60d at approximately 65 micromolg(-1) DW. Uronic acids were found in similar amounts in cell walls of both control and Cu-treated cells, whereas arabinose and galactose decreased to 61-67% in the presence of Cu. Cell wall polysaccharides were determined after successive extraction with 50mM CDTA, 50mM Na(2)CO(3), 1M KOH, and 4M KOH. The pectic fractions (CDTA- and Na(2)CO(3)-soluble) decreased to 47% and the hemicellulosic fractions (1M KOH- and 4M KOH-soluble) to 86% under Cu application. Approximately 43% of the Cu taken into cell walls was released following endo-pectate lyase treatment, suggesting that two-fifths of the total Cu accumulation was tightly bound to the homogalacturonan of the cell wall pectin.
本研究分析了铜（Cu）存在下，藓类 Scopelophila cataractae 原丝体和细胞壁基质多糖的生长动力学。在含有 0.2mM CuSO4 的培养基中培养的原丝体细胞迅速积累 Cu，在 30 至 60d 之间达到约 65µmol g-1 DW 的最大值。在对照和 Cu 处理的细胞细胞壁中均发现了相同量的糖醛酸，而阿拉伯糖和半乳糖在 Cu 存在下减少到 61-67%。细胞壁多糖用 50mM CDTA、50mM Na2CO3、1M KOH 和 4M KOH 进行连续提取后进行测定。在施加 Cu 后，果胶部分（CDTA 和 Na2CO3 可溶部分）减少到 47%，半纤维素部分（1M KOH 和 4M KOH 可溶部分）减少到 86%。内切果胶酶处理后，进入细胞壁的 Cu 约有 43%被释放，表明总 Cu 积累的五分之二与细胞壁果胶的同质半乳糖醛酸紧密结合。
