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开发一种多重实时 RT-PCR，可实现对甲型流感病毒的通用检测，并同时对甲型 H1N1/2009 病毒进行分型。

Development of a multiplex real-time RT-PCR that allows universal detection of influenza A viruses and simultaneous typing of influenza A/H1N1/2009 virus.

机构信息

West of Scotland Specialist Virology Centre, A Member of the UK Clinical Virology Network, Gartnavel General Hospital, Great Western Road, Glasgow G12 OYN, United Kingdom.

出版信息

J Virol Methods. 2010 Feb;163(2):258-61. doi: 10.1016/j.jviromet.2009.10.006. Epub 2009 Oct 23.

DOI:10.1016/j.jviromet.2009.10.006
PMID:19854220
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7173015/
Abstract

On June 11, 2009, the World Health Organization declared that the influenza A/H1N1/2009 virus had become the first influenza pandemic of the 21st century. Rapid detection and differentiation from seasonal and avian influenza would be beneficial for patient management and infection control. It was the aim of this study to develop a real-time RT-PCR that can detect all influenza A viruses and offer simultaneous typing for influenza A/H1N1/2009. This would be a useful addition to existing diagnostic protocols for influenza A. Its routine use would allow laboratories to screen out influenza A/H1N1/2009 positive samples rapidly and would reduce overall testing costs.

摘要

2009 年 6 月 11 日，世界卫生组织宣布甲型 H1N1/2009 流感病毒已成为 21 世纪的第一次流感大流行。快速检测和区分季节性流感和禽流感将有利于患者管理和感染控制。本研究的目的是开发一种能够检测所有甲型流感病毒并同时对甲型 H1N1/2009 进行分型的实时 RT-PCR。这将是对现有甲型流感诊断方案的有益补充。常规使用该方法可以使实验室快速筛选出甲型 H1N1/2009 阳性样本，并降低总体检测成本。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0302/7173015/c67090e821aa/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0302/7173015/c67090e821aa/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0302/7173015/c67090e821aa/gr1.jpg

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