Department of Chemical and Biological Engineering, Chongqing Institute of Technology, Chongqing, PR China.
Waste Manag Res. 2010 Apr;28(4):347-54. doi: 10.1177/0734242X09351180. Epub 2009 Oct 23.
The inhibition effects and mechanisms of chlorinated methane, anthraquinone and acetylene on methanogenesis in the anaerobic digestion process of biodegradable solid wastes were investigated. It was found that both chloroform and acetylene could effectively inhibit methanogens. Acetylene inhibited the activity of methanogens, while chloroform inhibited metabolic process of methanogenesis. A central composite design (CCD) and response surface regression analysis (RSREG) were employed to determine the optimum conditions and interaction effects of chloroform and acetylene in terms of methane and hydrogen production. Acetylene promoted the inhibition efficiency (F = 31.14; P < 0.01) more effectively than chloroform (F = 2.46; P > 0.05). In addition, a maximum hydrogen production of 1.6 ml was estimated under the optimum conditions of chloroform concentration of 6.69 mg kg(-1) and acetylene concentration of 3.08 x 10(-3) (v/v). Chloroform had a significant effect on enhancing the production of propionic acid and a minimum molar ratio of acetic acid to propionic acid of 0.707 was reached with the chloroform concentration of 9.24 mg kg(-1) and acetylene concentration of 4.0 x 10(-3) (v/v). Hence, methanogens can be inhibited while the stabilization process of solid wastes can still work well. Moreover, co-inhibition technology practice at landfills was feasible and the environmental damage was negligible, according to the analysis and experimental results.
研究了氯甲烷、蒽醌和乙炔对可生物降解固体废物厌氧消化过程中产甲烷作用的抑制效果和机制。结果表明，三氯甲烷和乙炔均可有效抑制产甲烷菌。乙炔抑制产甲烷菌的活性，而三氯甲烷则抑制产甲烷代谢过程。采用中心组合设计（CCD）和响应面回归分析（RSREG）确定了三氯甲烷和乙炔在甲烷和氢气生成方面的最佳条件和相互作用效应。乙炔比三氯甲烷（F = 31.14；P < 0.01）更有效地促进了抑制效率（F = 2.46；P > 0.05）。此外，在三氯甲烷浓度为 6.69 mg kg(-1)和乙炔浓度为 3.08 x 10(-3)（v/v）的最佳条件下，估计最大氢气产量为 1.6 ml。三氯甲烷对提高丙酸产量有显著影响，当三氯甲烷浓度为 9.24 mg kg(-1)和乙炔浓度为 4.0 x 10(-3)（v/v）时，达到了乙酸与丙酸的最小摩尔比 0.707。因此，在保持固体废物稳定化过程的同时，可以抑制产甲烷菌。根据分析和实验结果，在垃圾填埋场实施共抑制技术是可行的，环境破坏可以忽略不计。
