热纤梭菌产家族 10 木聚糖酶的糖基化和额外结构域对其热稳定性的影响。
Effect of glycosylation and additional domains on the thermostability of a family 10 xylanase produced by Thermopolyspora flexuosa.
机构信息
Department of Biotechnology and Chemical Technology, Aalto University, P.O. Box 6100, 02015 TKK, Espoo, Finland.
出版信息
Appl Environ Microbiol. 2010 Jan;76(1):356-60. doi: 10.1128/AEM.00357-09. Epub 2009 Oct 23.
Abstract
The effects of different structural features on the thermostability of Thermopolyspora flexuosa xylanase XYN10A were investigated. A C-terminal carbohydrate binding module had only a slight effect, whereas a polyhistidine tag increased the thermostability of XYN10A xylanase. In contrast, glycosylation at Asn26, located in an exposed loop, decreased the thermostability of the xylanase. The presence of a substrate increased stability mainly at low pH.
摘要
研究了不同结构特征对嗜热解纤维梭菌木聚糖酶 XYN10A 热稳定性的影响。C 端碳水化合物结合模块的影响很小，而多组氨酸标签则提高了 XYN10A 木聚糖酶的热稳定性。相比之下，位于暴露环中的 Asn26 位的糖基化降低了木聚糖酶的热稳定性。底物的存在主要在低 pH 值下增加稳定性。
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