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Engineering the thermostability of a TIM-barrel enzyme by rational family shuffling.通过合理的家族改组工程化改造TIM桶状酶的热稳定性。
Biochem Biophys Res Commun. 2008 Oct 3;374(4):725-30. doi: 10.1016/j.bbrc.2008.07.095. Epub 2008 Jul 27.
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Modulation of protein stability by O-glycosylation in a designed Gc-MAF analog.在一种设计的Gc-MAF类似物中，O-糖基化对蛋白质稳定性的调节作用。
Biophys Chem. 2008 May;134(3):157-67. doi: 10.1016/j.bpc.2008.02.005. Epub 2008 Feb 21.
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Effect of family 22 carbohydrate-binding module on the thermostability of Xyn10B catalytic module from Clostridium stercorarium.
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Fusion of carbohydrate binding modules from Thermotoga neapolitana with a family 10 xylanase from Bacillus halodurans S7.嗜热栖热袍菌碳水化合物结合模块与嗜碱芽孢杆菌S7的10家族木聚糖酶的融合
Extremophiles. 2007 Jan;11(1):169-77. doi: 10.1007/s00792-006-0023-4. Epub 2006 Sep 28.
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Probing the structural basis for the difference in thermostability displayed by family 10 xylanases.探究10家族木聚糖酶所表现出的热稳定性差异的结构基础。
J Mol Biol. 2006 Jun 30;360(1):157-67. doi: 10.1016/j.jmb.2006.05.002. Epub 2006 May 15.
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The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling.SWISS-MODEL工作区：一个用于蛋白质结构同源建模的基于网络的环境。
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Reclassification of Nonomuraea flexuosa (Meyer 1989) Zhang et al. 1998 as Thermopolyspora flexuosa gen. nov., comb. nov., nom. rev.弯曲野野村菌（Meyer，1989）Zhang等人，1998年重新分类为弯曲嗜热多孢菌，新属，新组合，名称修订
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Deglycosylation of glucoamylase from Aspergillus niger: effects on structure, activity and stability.黑曲霉葡萄糖淀粉酶的去糖基化作用：对结构、活性和稳定性的影响
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Thermostable xylanases, Xyn10A and Xyn11A, from the actinomycete Nonomuraea flexuosa: isolation of the genes and characterization of recombinant Xyn11A polypeptides produced in Trichoderma reesei.来自挠曲野野村放线菌的耐热木聚糖酶Xyn10A和Xyn11A：基因的分离及里氏木霉中产生的重组Xyn11A多肽的特性分析
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The use of forced protein evolution to investigate and improve stability of family 10 xylanases. The production of Ca2+-independent stable xylanases.
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热纤梭菌产家族 10 木聚糖酶的糖基化和额外结构域对其热稳定性的影响。

Effect of glycosylation and additional domains on the thermostability of a family 10 xylanase produced by Thermopolyspora flexuosa.

机构信息

Department of Biotechnology and Chemical Technology, Aalto University, P.O. Box 6100, 02015 TKK, Espoo, Finland.

出版信息

Appl Environ Microbiol. 2010 Jan;76(1):356-60. doi: 10.1128/AEM.00357-09. Epub 2009 Oct 23.

DOI:10.1128/AEM.00357-09
PMID:19854928
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2798631/
Abstract

The effects of different structural features on the thermostability of Thermopolyspora flexuosa xylanase XYN10A were investigated. A C-terminal carbohydrate binding module had only a slight effect, whereas a polyhistidine tag increased the thermostability of XYN10A xylanase. In contrast, glycosylation at Asn26, located in an exposed loop, decreased the thermostability of the xylanase. The presence of a substrate increased stability mainly at low pH.

摘要

研究了不同结构特征对嗜热解纤维梭菌木聚糖酶 XYN10A 热稳定性的影响。C 端碳水化合物结合模块的影响很小，而多组氨酸标签则提高了 XYN10A 木聚糖酶的热稳定性。相比之下，位于暴露环中的 Asn26 位的糖基化降低了木聚糖酶的热稳定性。底物的存在主要在低 pH 值下增加稳定性。