Han Liying, Pansare Vaishali, Al-Abbadi Mousa, Husain Mujtaba, Feng Jining
Department of Pathology, Wayne State University and Karmanos Cancer Institute, Detroit, Michigan, USA.
Diagn Cytopathol. 2010 May;38(5):333-6. doi: 10.1002/dc.21202.
Malignant ascites may be the first presentation of an unsuspected cancer. Pancreas and ovary are among the organs that are usually evaluated as a source of primary. The purpose of this study is to investigate a panel of immunohistochemical stains to help differentiate pancreatic from ovarian carcinoma. We evaluated the immunohistochemical staining of eight commercially available antibodies MUC1, MUC2, MUC5ac, Wilm's tumor susceptibility gene 1 (WT1), cytokeratin 7 (CK7), CK20, CA125, and CA19.9 in 25 effusion specimens with evidence of metastatic carcinoma including 14 ovarian serous carcinomas, 9 pancreatic adenocarcinomas, and 2 unknown primaries. Primary ovarian serous carcinomas were positive for WT-1 (100%), CK7 (93%), CK20 (43%), CA125 (100%), CA19.9 (50%), MUC1 (100%), MUC2 (0%), and MUC5ac (0%). Primary pancreatic carcinomas were positive for MUC5ac (100%), MUC1 (100%), CA19.9 (100%), CK7 (78%), CK20 (22%), CA125 (89%), WT-1 (0%), and MUC 2 (0%). The combination of MUC5ac positivity/WT-1 negativity was seen in 100% of pancreatic carcinoma, whereas MUC5ac negativity/WT-1 positivity in 100% of ovarian serous carcinoma. It appears that the combination of MUC5ac and WT-1 stains is useful in distinguishing pancreatic ductal from ovarian serous carcinoma in body fluid cytology.
恶性腹水可能是未被怀疑的癌症的首发表现。胰腺和卵巢是通常被评估为原发灶来源的器官。本研究的目的是研究一组免疫组织化学染色，以帮助区分胰腺癌和卵巢癌。我们评估了8种市售抗体MUC1、MUC2、MUC5ac、威尔姆斯瘤易感基因1（WT1）、细胞角蛋白7（CK7）、CK20、CA125和CA19.9在25例有转移癌证据的积液标本中的免疫组织化学染色情况，其中包括14例卵巢浆液性癌、9例胰腺腺癌和2例原发灶不明的病例。原发性卵巢浆液性癌WT-1阳性率为100%，CK7为93%，CK20为43%，CA125为100%，CA19.9为50%，MUC1为100%，MUC2为0%，MUC5ac为0%。原发性胰腺癌MUC5ac阳性率为100%，MUC1为100%，CA19.9为100%，CK7为78%，CK20为22%，CA125为89%，WT-1为0%，MUC2为0%。100%的胰腺癌可见MUC5ac阳性/WT-1阴性组合，而100%的卵巢浆液性癌可见MUC5ac阴性/WT-1阳性组合。在体液细胞学中，MUC5ac和WT-1染色的组合似乎有助于区分胰腺导管癌和卵巢浆液性癌。
