Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev str. Bl. 9, 1113 Sofia, Bulgaria.
Phytochem Anal. 2010 Mar-Apr;21(2):186-91. doi: 10.1002/pca.1176.
Because of its chemical diversity, the only way to standardise propolis is to specify multiple standards for different propolis types according to the corresponding chemical profile. So far, this has been done only for European propolis.
To develop a rapid low-cost spectrophotometric procedure for quantification of bioactive prenylated flavanones in Taiwanese propolis.
The proposed method quantifies the total flavanones on the basis of their absorption as coloured phenylhydrazones formed by interaction with 2,4-dinitrophenylhydrazine. The procedure was validated through model mixture of compounds representing the composition of Taiwanese propolis according to previous studies. The major flavanones of the propolis samples (propolins C, D, F and G) were quantified by HPLC. Antiradical activity against DPPH was also measured. The DNP (dinitrophenylhydrazine) spectrophotometric method is applied for the first time for quantification of prenylated flavanones.
Spectophotometric procedure applicable to new type propolis (Macaranga type) was developed with recovery between 105 and 110% at the concentration range of 0.573-1.791 mg/mL. Six propolis samples were analysed by spectrophotometry using the procedure developed and validated, and by HPLC as the results demonstrated satisfactory agreement. Neither the spectrophotometric data nor the values measured by HPLC showed significant correlation with the antiradical activity against DPPH.
The proposed spectrophotometric procedure is useful for routine analyses of Macaranga-type propolis, because of its simplicity, repeatability and acceptable accuracy. Its application to a number of commercial samples could be used as a basis for standardisation and quality control of Pacific propolis.
由于其化学多样性，标准化蜂胶的唯一方法是根据相应的化学特征为不同类型的蜂胶指定多个标准。到目前为止，这仅针对欧洲蜂胶进行了研究。
开发一种快速、低成本的分光光度法，用于定量测定台湾蜂胶中的生物活性prenylated flavanones。
该方法基于与 2,4-二硝基苯肼相互作用形成有色苯腙的物质的吸收来定量总 flavanones。该方法是根据以前的研究，通过代表台湾蜂胶成分的化合物模型混合物进行验证的。通过 HPLC 定量测定蜂胶样品中的主要 flavanones（propolin C、D、F 和 G）。还测量了对 DPPH 的抗自由基活性。首次将 DNP（二硝基苯肼）分光光度法应用于 prenylated flavanones 的定量。
开发了适用于新型蜂胶（Macaranga 型）的分光光度法，在 0.573-1.791 mg/mL 的浓度范围内回收率为 105-110%。使用开发和验证的程序以及 HPLC 对 6 个蜂胶样品进行分光光度法分析，结果表明两者之间存在令人满意的一致性。分光光度数据和 HPLC 测量值均与对 DPPH 的抗自由基活性没有显著相关性。
该分光光度法程序简单、可重复性好、准确度可接受，可用于常规分析 Macaranga 型蜂胶。其对许多商业样品的应用可以作为太平洋蜂胶标准化和质量控制的基础。
