抗IgA荧光微球免疫测定法的开发与验证

Development and validation of a fluorescent microsphere immunoassay for anti-IgA.

作者信息

Rumilla K M, Winters J L, Peterman J M, Jedynak E A, Homburger H A

机构信息

Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.

出版信息

Immunohematology. 2009;25(1):24-8.

PMID:19856730

Abstract

Anti-IgA may cause anaphylactic transfusion reactions in IgA-deficient individuals. Testing for IgG anti-IgA is useful to identify persons at risk. This report describes an immunoassay for anti-IgA that uses polyclonal IgA coupled to fluorescent microspheres as an immunosorbent. Anti-IgA is detected by phycoerythrin-labeled anti-IgG. The assay is calibrated in arbitrary units by use of a serum that contains anti-IgA. Dose-response studies with sera that contain anti-IgA showed positive responses at dilutions up to 32-fold greater than the dilution used to test patients' samples. Inhibition studies with purified IgA and IgA-deficient serum showed no inhibition with IgA-deficient serum and complete inhibition with soluble IgA. Clinical tests performed in more than 90 assays had a CV of 13.6 percent for measurements of an internal positive control. The fluorescent immunoassay method is rapid, reproducible, and sensitive to low concentrations of IgG anti-IgA.

摘要

抗 IgA 可能会在 IgA 缺乏个体中引发过敏性输血反应。检测 IgG 抗 IgA 有助于识别有风险的人群。本报告描述了一种抗 IgA 免疫测定法，该方法使用偶联荧光微球的多克隆 IgA 作为免疫吸附剂。抗 IgA 通过藻红蛋白标记的抗 IgG 进行检测。该测定法使用含有抗 IgA 的血清以任意单位进行校准。用含有抗 IgA 的血清进行的剂量反应研究表明，在稀释度比用于检测患者样本的稀释度高 32 倍时仍显示阳性反应。用纯化 IgA 和 IgA 缺乏血清进行的抑制研究表明，IgA 缺乏血清无抑制作用，而可溶性 IgA 有完全抑制作用。在 90 多次测定中进行的临床测试，对内部阳性对照测量的变异系数为 13.6%。荧光免疫测定法快速、可重复，且对低浓度的 IgG 抗 IgA 敏感。