Eckrich R J, Mallory D M, Sandler S G
National Reference Laboratory for Blood Group Serology, American Red Cross Blood Services, Rockville, Maryland.
Transfusion. 1993 Jun;33(6):488-92. doi: 10.1046/j.1537-2995.1993.33693296811.x.
Four methods were compared as to their suitability for excluding IgA deficiency in the investigation of suspected anti-IgA transfusion reactions. The methods were radial immunodiffusion, passive hemagglutination inhibition, sandwich enzyme-linked immunosorbent assay, and membrane enzyme immunoassay. Parallel testing was performed on sera from 40 patients or blood donors previously found to have anti-IgA and low or undetectable levels of IgA. All test methods identified the 40 sera as having abnormally low IgA levels. The membrane enzyme immunoassay required 10 minutes or less for testing, as compared to 3 hours for passive hemagglutination inhibition, 4 hours for sandwich enzyme-linked immunosorbent assay, and 48 hours for radial immunodiffusion. The membrane enzyme immunoassay offers the potential for a rapid, instrument-free screen of IgA levels and therefore may be useful in identifying those patients with suspected anti-IgA anaphylactic transfusion reactions who are not IgA deficient and do not require IgA-deficient blood components for additional transfusions.
在疑似抗IgA输血反应的调查中，对四种排除IgA缺乏症的方法的适用性进行了比较。这些方法分别是放射免疫扩散法、被动血凝抑制法、夹心酶联免疫吸附测定法和膜酶免疫测定法。对先前发现有抗IgA且IgA水平低或检测不到的40名患者或献血者的血清进行了平行检测。所有检测方法均鉴定出这40份血清的IgA水平异常低。膜酶免疫测定法检测需要10分钟或更短时间，相比之下，被动血凝抑制法需要3小时，夹心酶联免疫吸附测定法需要4小时，放射免疫扩散法需要48小时。膜酶免疫测定法有可能快速、无需仪器地筛查IgA水平，因此可能有助于识别那些疑似抗IgA过敏输血反应但并非IgA缺乏且不需要输注IgA缺乏血液成分的患者。
