Department of Animal & Dairy Science, University of Georgia, Athens, Georgia 30602-2771, USA.
J Med Food. 2009 Oct;12(5):959-66. doi: 10.1089/jmf.2008.0262.
The effect of octanoate and decanoate, respectively, eight- and 10-carbon medium-chain fatty acids (MCFAs), on apoptotic signaling in 3T3-L1 adipocytes was investigated. 3T3-L1 adipocytes were treated with various concentrations of octanoate or decanoate. Cell viability, apoptosis, and expression of apoptosis-related proteins were investigated. Results indicated that both octanoate and decanoate decreased viability, increased apoptosis, and increased reactive oxygen species production. Immunoblotting analysis showed an increase in the levels of cytoplasmic cytochrome c and cleaved poly(ADP-ribose) polymerase by octanoate and decanoate. Concomitantly, we observed that pro-caspase-3 was decreased, resulting in the induced accumulation of the cleaved form of caspase-3 by both octanoate and decanoate. In addition, both octanoate and decanoate increased the expression of pro-apoptotic Bax with an accompanied decrease of anti-apoptotic Bcl-2. These results show that octanoate and decanoate mediate adipocyte apoptosis via a caspase-dependent mitochondrial pathway in 3T3-L1 adipocytes. MCFAs thus decrease adipocyte number by initiating the apoptotic process in 3T3-L1 adipocytes.
分别研究了辛酸盐和癸酸盐这两种 8 碳和 10 碳中链脂肪酸（MCFA）对 3T3-L1 脂肪细胞凋亡信号的影响。用不同浓度的辛酸盐或癸酸盐处理 3T3-L1 脂肪细胞。研究了细胞活力、细胞凋亡和与凋亡相关的蛋白表达。结果表明，辛酸盐和癸酸盐均降低细胞活力、增加细胞凋亡并增加活性氧的产生。免疫印迹分析表明，辛酸盐和癸酸盐增加了细胞质细胞色素 c 和聚（ADP-核糖）聚合酶的裂解水平。同时，我们观察到原胱天蛋白酶-3减少，导致辛酸盐和癸酸盐均诱导 caspase-3 的裂解形式积累。此外，辛酸盐和癸酸盐均增加了促凋亡 Bax 的表达，同时抗凋亡 Bcl-2 减少。这些结果表明，辛酸盐和癸酸盐通过 3T3-L1 脂肪细胞中的 caspase 依赖性线粒体途径介导脂肪细胞凋亡。MCFA 通过启动 3T3-L1 脂肪细胞中的凋亡过程来减少脂肪细胞数量。
